Human CD4+ T cell clone longevity in tissue culture: lack of influence of donor age or cell origin. |
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| Authors: | Graham Pawelec Yvonne Barnett Erminia Mariani Rafael Solana |
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| Affiliation: | 1. Gynecologic Oncology Program, AdventHealth Cancer Institute, Orlando, FL 32804, USA;2. Office of Clinical Research, AdventHealth Cancer Institute, Orlando, FL 32804, USA;1. Shanghai Tenth People''s Hospital, Tongji University School of Medicine, Shanghai 200072, China;2. Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute at Frederick, Frederick, MD 21702, USA;3. Laboratory of Inflammation Biology, Department of Biological Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061-0910, USA;4. Basic Research Program, Leidos Biomedical Research, Inc., Frederick, MD 21702, USA |
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| Abstract: | CD4+ human T cell clones were derived from activated peripheral blood lymphocytes of healthy young adults to establish cloning efficiencies (CE) and clonal longevities. These results were compared with those obtained using cells from the very elderly, also in excellent health. CE and both maximal and average longevities under appropriate culture conditions were very similar in the two groups. Moreover, CE of CD34+ hematopoietic progenitor cells and longevities of clones derived from them were also similar. Finally, CE and longevities of clones derived from a patient with chronic myelogenous leukaemia were found to be comparable as well. Hence, T cells with absolutely no antigenic exposure in vivo prior to cloning (i.e. CD34-derived) and those potentially exposed to chronic antigenic stimulation (CML-derived) and those from young or old donors all had similar cloning and propagation properties in vitro. These results imply that the longevity of T cells in culture is more likely to be dictated by cloning conditions than any intrinsic differences between the cells studied. |
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