| UPLC-PDA-ESI/MS分析荭草中化学成分 |
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| 引用本文： | 黄勇,郑林,何峰,李勇军,王爱民,王永林. UPLC-PDA-ESI/MS分析荭草中化学成分[J]. 中国中药杂志, 2011, 36(1): 57-59 |
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| 作者姓名： | 黄勇 郑林 何峰 李勇军 王爱民 王永林 |
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| 作者单位： | 贵阳医学院药学院,贵州,贵阳,550004 |
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| 基金项目： | 国家自然科学基金项目(30860366);国家科技重大专项课题(2008ZX09101-021);贵州省科技计划重大专项项目(黔科合重大专项字[2007]6010号) |
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| 摘 要： | 目的:建立荭草药材中化学成分的超高效液相色谱-电喷雾质谱(UPLC-PDA-ESI/MS)分析方法.方法:采用ACQUITY UPLC系统,BEH C18色谱柱(2.1mm×100 mm,1.7μm),乙腈-0.1%甲酸水溶液为流动相,流速为0.3 mL·min-1,梯度洗脱,检测波长190～400nm,柱温45 ℃.Waters电喷雾三重四级质谱仪,负离子检测模式,ESI喷雾电压3 kV,去溶剂气温度350 ℃,去溶剂气流量750 L·h-1(N2),锥孔气流量50 L·h-1(N2),扫描范围m/z 100～1000.结果:荭草中化学成分获得了较好的分离和检测,共鉴定出1个酚酸类成分和11个黄酮类成分.结论:方法灵敏度高、分离度好,适用于荭草药材中化学成分的快速定性鉴定.
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| 关 键 词： | 荭草 酚酸 黄酮 UPLC-PDA-ESI/MS |
| 收稿时间： | 2010-08-15 |
Identification of compounds in herb of Polygonum orientale by UPLC-PDA-ESI/MS |
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| HUANG Yong,ZHENG Lin,HE Feng,LI Yongjun,WANG Aimin and WANG Yonglin. Identification of compounds in herb of Polygonum orientale by UPLC-PDA-ESI/MS[J]. China Journal of Chinese Materia Medica, 2011, 36(1): 57-59 |
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| Authors: | HUANG Yong ZHENG Lin HE Feng LI Yongjun WANG Aimin WANG Yonglin |
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| Affiliation: | School of Pharmacy, Guiyang Medical College, Guiyang 550004,China;School of Pharmacy, Guiyang Medical College, Guiyang 550004,China;School of Pharmacy, Guiyang Medical College, Guiyang 550004,China;School of Pharmacy, Guiyang Medical College, Guiyang 550004,China;School of Pharmacy, Guiyang Medical College, Guiyang 550004,China;School of Pharmacy, Guiyang Medical College, Guiyang 550004,China |
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| Abstract: | |
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| Keywords: | Polygonum orientale phenolic acid flavonoids UPLC-PDA-ESI/MS |
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