维生素D受体基因多态性对中国藏族肺结核病人排菌情况的影响

目的探讨维生素D受体(VDR)基因多态性与中国藏族结核病人群抗酸杆菌检测结果的关系。方法采用病例对照研究设计,用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)对45例菌阳病人和90例菌阴病人进行VDR基因分型,检测VDR基因中Fok1与Taq1多态性,分析两种多态性与痰结核杆菌培养及抗酸染色之间的关系。结果VDR-FF、VDR-Ff及VDR-ff三种基因型在菌阳组和菌阴组的分布频率分别为36%、40%、24%和59%、33%、8%,其中ff基因型在菌阳组中的频率显著高于菌阴组,OR值95%CI为3.836(1.372,10.719);VDR-TT及VDR-Tt两种基因型在菌阳组和菌阴组的分布频率分别为96%、4%和94%、6%,两组比较无显著性差异(P>0.05)。结论VDR-ff基因型可能是中国藏族菌阳肺结核病人的易感基因型。

The relevance of vitamin D receptor gene polymorphisms to bacteriological yeilds in Chinese Tibetan patients with pulmonary tuberculosis

Objective To explore the association between polymorphisms of vitamin D receptor(VDR) gene and positive yield of acid-fast bacilli in sputum.Methods Case-control study was conducted in the(Chinese) Tibetan population.VDR Fok1 and Taq1 polymorphisms were compared among 45 patients with pulmonary tuberculosis(TB) with positive yield of acid-fast bacilli and 95 patients with negative yields by PCR-RFLP technique.Associations of VDR polymorphisms with the sputum mycobacterial culture and auramine stain were analyzed.Results The genotype frequencies of VDR-FF,VDR-Ff,VDR-ff were 36%,40%,24% in those with positive yield and 59%,33%,8% in those with negative yield,respectively.A significant increase of VDR genotype ff(mutant homozygotes) was revealed in those with positive yield when compared to those with negative yield(P<0.01,OR 3.836;95% confidence interval,1.372,10.719).The genotype frequencies of VDR-TT,VDR-Tt were 96%,4% in positive yield group and 94%,6% in negative yield group,respectively.No significant difference in these genotype frequencies was observed between positive and negative subjects(P>0.05).(Conclusion) The VDR-ff genotype might be associated with the pulmonary tuberculosis with positive bacterial yield in Chinese Tibetan population.