| 乳酸脱氢酶同工酶C_4与男性不育的关系 |
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| 引用本文： | 何剑秋,金辛良,周孝瑚,余振珏,姚孟树.乳酸脱氢酶同工酶C_4与男性不育的关系[J].复旦学报(医学版),1993(5). |
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| 作者姓名： | 何剑秋 金辛良 周孝瑚 余振珏 姚孟树 |
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| 作者单位： | 上海医科大学基础医学院组织胚胎学教研室
(何剑秋，周孝瑚，余振珏)，上海市闸北区中心医院泌尿外科
(金辛良)，华山医院泌尿外科(姚孟树) |
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| 基金项目： | 高等学校科学基金委员会博士点基金(858256/210146) |
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| 摘 要： | 用自制的抗人乳酸脱氢酶同工酶C_4(LDH-C_4)单克隆抗体(McAb),以免疫荧光、BA-ELISA及聚丙烯酰胺凝胶电泳(PAGE)等方法,测定了110例正常及各种不育症患者精子及精浆中LDH-C_4的含量。结果表明:不明原因性不育症、少精症及无精症患者精液中LDH-C_4的含量明显低于正常对照组,与正常组比较P值均<0.001,有极显著性差异;免疫荧光还发现不育症组伴有各种畸形精子。LDH-C_4含量的明显降低可能与男性不育有关。
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| 关 键 词： | 乳酸脱氢酶同工酶C_4 免疫荧光 BA-ELISA 聚丙烯酰胺凝胶电脉 精液 |
RELATIONSHIP BETWEEN LACTATE DEHYDROGENASE ISOZYME-C_4 AND MALE STERILITY |
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| He Jian-Qiu,Jin Xin-Liang,Zhou Xiao-Hu,She Zhen-Jue,Yao Meng-Shu.RELATIONSHIP BETWEEN LACTATE DEHYDROGENASE ISOZYME-C_4 AND MALE STERILITY[J].Fudan University Journal of Medical Sciences,1993(5). |
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| Authors: | He Jian-Qiu Jin Xin-Liang Zhou Xiao-Hu She Zhen-Jue Yao Meng-Shu |
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| Abstract: | Using anti-human lactate dehydrogenase isozgme-O4 (LDH-O4) monoclonal antibody(McAb)with immunofluorescen.ee and BA-ELISA assay and polyacrylamide electrophoresis, the LDH-C4 content in the sperm and seminal plasma of 110 normal men and various male sterility patients were determined. Results showed that LDH-C4 contents in the semen of sterility patients of unknown causes, and azoospermia or oligospermia patients were remarkably lower than those in the normal control group, P<0.001.Immunofluoresconce also showed the existence of abnormal sperms. Consequently, the remarkeblly low LDH-C4 content might represent one of the important causes of male sterility. |
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| Keywords: | laetate dehydrogenase isozyme-C_4 immunofluorescence BAELISA polyacrylamide electrophorasis semen |
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