Immunochemistry of apamin—bee venom neurotoxin—I. Radioimmunoassay with apamin and its derivatives |
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| Authors: | Serguei V Komissarenko Serguei V Vasilenko Elena G Elyakova Elena A Surina Anatolij I Miroshnikov |
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| Institution: | 1. A. V. Palladin Institute of Biochemistry, Kiev, U.S.S.R.;2. M. M. Shemyakin Institute of Bioorganic Chemistry, Moscow, U.S.S.R. |
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| Abstract: | Antibodies against apamin, a neurotoxic polypeptide from bee venom were raised in rabbits by immunization with apamin or apamin-BSA conjugates. 3H-apamin or 125I-apamin were used in radioimmunoassay with anti-apamin for the detection of the apamin antigenic site. The inhibitory activity toward the labelled apamin-anti-apamin binding was maximal with unlabelled apamin and decreased in the range: apamin > Cys1, Lys4-disuccinilated apamin > Cys1, Lys4-diacetylated apamin > Cys1, Lys4-diacetylated apamin with carboxymethylated His18. Dipyrimidyl-Orn13,Orn14-apamin derivative almost had no inhibitory activity on labelled apamin binding emphasizing that Arg13, Arg14 are the most essential for the apamin topographic antigenic site. |
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| Keywords: | Correspondence should be addressed to: Dr S V Komissaernko Chief Laboratory of Immunochemistry A V Palladin Institute of Biochemistry 9 Leontovicha str Kie-30 U S S R |
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