HPLC-电化学检测法用于黄芩苷和黄芩素大鼠血浆稳定性研究

目的：考察黄芩苷（BG）和黄芩素（B）在缓冲液及大鼠血浆中的稳定性，建立测定二者在大鼠血浆中含量的样品处理方法，并初步探讨二者在血浆中稳定性的影响因素。方法：采用HPLC-电化学检测法，分别考察BG，B在不同条件下的稳定性及使二者稳定的条件。结果：BG，B在大鼠血浆中较在pH7．4的缓冲液中更不稳定。在pH7．4缓冲液中加入抗氧化剂可使BG和B的稳定性明显提高，剩余百分含量达到102％和100％；在大鼠血浆中加入抗氧化剂及1mol·L^-1。HCl可使BG和B的稳定性明显增加，剩余百分含量可分别达到98％和103％。结论：BG和B在pH7．4缓冲液中的稳定性主要受氧化影响，BG和B在血浆中的稳定性不仅与氧化有关，还受血浆中的内源物质影响，最终确定每1mL大鼠血浆中加入抗氧剂及100μL 1mol·L^-1HCl为血浆样品的处理条件，此条件可使样品稳定。

Study on stability of baicalin and baicalein in mouse plasma by HPLC-ECD

Objective: To compare the stability of baicalin and baicalein in buffered aqueous solutions and mouse plasma in order to discuss the degradation mechanism and the method of disposing samples.Method: HPLC-ECD was used to determine baicalin and baicalein in different conditions,and the disposing samples methods were selected to stabilize them.Result: Baicalin and baicalein in the buffered aqueous solution at pH 7.4 was more stable than that in the mouse plasma.After adding Vit C in the buffered aqueous soluti...