Serological characterization of occult hepatitis B virus infection among blood donors in India |
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| Authors: | Veena Doda Satyam Arora Tapannidhi Kirtania |
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| Affiliation: | 1. OB/GYN Dept., Faculty of Medicine, Minia University, Minia, Egypt;2. OB/GYN Dept., Taiba Hospital, Kuwait;3. Assistant Professor of Pathology, Faculty of Medicine, Minia University, Minia, Egypt;4. Consultant Pathologist, Taiba Hospital, Kuwait;1. Hépatologie gastroentérologie et nutrition pédiatrique, Hospices Civils de Lyon, Hôpital Femme-Mère-Enfant, F-69500 Bron, France;2. Centre de référence national pour la maladie de Wilson, Hospices Civils de Lyon, Hôpital Femme-Mère-Enfant, F-69500 Bron, France;3. Univ Grenoble Alpes, BIG-LCBM, F-38000 Grenoble, France;4. CNRS, BIG-LCBM, F-38000 Grenoble, France;5. CEA, BIG-LCBM-BIOMET, F-38000 Grenoble, France;6. Service central d’anatomie et cytologie pathologiques, Hospices Civils de Lyon, Hôpital Edouard Herriot, F-69003 Lyon (moved to Centre de Biologie et Pathologie Est, F-69677 Bron since september 2017), France;7. Laboratoire d’Analyse de Trace, Biochimie et biologie moléculaire, Hospices Civils de Lyon, Hôpital Edouard Herriot, F-69003 Lyon (moved to Centre de Biologie et d’AnatomoPathologie Sud, F-69495 Pierre-Bénite since september 2016), France;1. Center for Surgical Outcomes Research, Nationwide Children''s Hospital, Columbus, Ohio;2. Department of Pediatric Surgery, Nationwide Children''s Hospital, Columbus, Ohio;3. Department of Pediatric Radiology, Nationwide Children''s Hospital, Columbus, Ohio;1. Infectious Diseases Laboratory, YRG Centre for AIDS Research and Education (YRG CARE), Voluntary Health Services Hospital Campus, Taramani, Chennai, India;1. AP–HP, hôpital Pitié-Salpêtrière, service d’hépato-gastroentérologie, unité médicale de transplantation hépatique, 75013 Paris, France;2. Sorbonne universités, UPMC université Paris 06, Inserm, UMR_S 938, CDR Saint-Antoine, institute of cardiometabolism and nutrition (ICAN), Paris, France;3. AP–HP, Pitié-Salpêtrière university hospital, department of genetics and reference center for adult neurometabolic diseases, Paris, France;4. Sorbonne université, UPMC-Paris 6, UMR S 1127, Inserm U 1127, CNRS UMR 7225, and ICM, 75013, Paris, France |
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| Abstract: | IntroductionDiscovery of hepatitis B infections characterized by the presence of viral genome without detectable HBsAg (Occult Hepatitis; OBI) has initiated a considerable amount of research in this regard. Our study is a serological and molecular characterization of OBI among the donors who donated at our blood bank during the study period.Material and MethodDuring the study period HBsAg ELISA non reactive ID-NAT reactive donors samples were screened for presence of antibody against HBc, HBs and HBe. Molecular analysis of these NAT yield samples was undertaken for detection of the viral load and genotyping.ResultWe studied 28,134 HBsAg ELISA non reactive donor samples. On testing them with ID-NAT, HBV DNA was detected in 25 samples. Eighteen samples out of these 25 NAT yield were further worked up. The 66.6% of the NAT yield samples (12 out of 18) were reactive for antibody against HBc. The 25% (3 out of 12) of these NAT yield samples having antibody against core antigen also had antibody against HBs. The 27.7% (5 out of 18) of NAT yield detected by ID-NAT did not have any detectable serological marker in blood. Four out of 12 core antibody positive NAT yield samples had genotype A HBV infection.ConclusionAs per our study molecular detection of HBV DNA by ID-NAT, we were able to analyze 18 HBV NAT yield cases among 28,134 HBsAg ELISA non reactive donors. Out of 18, 12 donors were OBI whereas the rest (6) were in window period (WP yield) of HBV infection. One out of every 3.6 NAT yield detected by ID-NAT was non reactive for all serological markers. |
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