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Development of polymorphic microsatellite markers for the microendemic pupfishes Cyprinodon julimes and C. pachycephalus |
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| Authors: | Evan W. Carson Rochelle R. Beasley Kenneth L. Jones Stacey L. Lance Ma de Lourdes Lozano-Vilano Lilia Vela-Valladares Iris Banda-Villanueva Thomas F. Turner Mauricio De la Maza-Benignos |
| Affiliation: | 1. Department of Biology and Museum of Southwestern Biology, University of New Mexico, Albuquerque, NM, 87131, USA 2. Savannah River Ecology Laboratory, University of Georgia, Aiken, SC, 29802, USA 3. Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, CO, 80045, USA 5. Laboratorio de Ictiología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, 66450, San Nicolás de los Garza, NL, Mexico 4. Pronatura Noreste, A.C, Loma Larga 235, Col. Loma Larga, 64710, Monterrey, NL, Mexico |
| Abstract: | We developed microsatellite loci for the Julimes pupfish, Cyprinodon julimes. Twenty-five loci were screened across 19 individuals from Julimes Spring, Chihuahua, Mexico. The number of alleles per locus ranged from 2 to 14, observed heterozygosity ranged from 0.105 to 0.947, and the probability of identity values ranged from 0.022 to 0.588. We then tested for cross-amplification in the bighead pupfish, C. pachycephalus; twenty-three individuals from San Diego de Alcalá, Chihuahua, Mexico, were screened across the 20 loci that amplified cleanly. These new loci will be used for long-term genetic monitoring of these critically endangered species. |
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