The effect of TRAIL molecule on cell viability in <Emphasis Type="Italic">in vitro</Emphasis> beta cell culture |
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Authors: | I Tekmen D Özyurt Ç Pekçetin Z Buldan |
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Institution: | (1) Department of Histology and Embryology, Dokuz Eylul University Medical Faculty, 35340 Inciralti, Izmir, Turkey |
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Abstract: | Insulin-dependent diabetes mellitus (IDDM) is an organ-specific autoimmune disorder triggered by autoreactive T cells directed
to pancreas beta-cell antigens. In this disorder, more than 90% of beta cells are destroyed. Cell death may be mediated via
soluble or membrane-bound cell death ligands. One of these ligands may be tumour necrosis factor (TNF)-related apoptosis-inducing
ligand (TRAIL), a member of the TNF-α superfamily. In the present study, we examined whether TRAIL had cytotoxic effects on
adult rat pancreas beta cell cultures and INS1-E rat insulinoma cell line cultures or not. In this study, cell destruction
models were built with TRAIL concentrations of 10, 100 and 1000 ng. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
(MTT) test was used for evaluating cell viability. It was detected that cell cultures with TRAIL added showed no differences
statistically when compared with control cultures containing no toxic additions. These results showed that TRAIL did not have
significant cytotoxic effects on pancreas beta cell culture and INS-1E rat insulinoma cell line cultures. Detection of the
expression of TRAIL receptors and natural apoptosis inhibitor proteins will be favourable to investigate the resistance mechanisms
to TRAIL-induced cell death in this cell culture system. |
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Keywords: | Type 1 diabetes mellitus INS-1E cell line Cytotoxicity TRAIL MTT |
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