修复关节软骨大面积缺损的实验研究

目的 比较和评价筋膜软骨细胞和骨膜、关节软骨移植修复关节软骨大面积缺损的能力和生物特性。方法 用冻存和新鲜的异体筋膜上培养的软骨细胞、骨膜和关节软骨移植修复大面积关节软骨缺损,通过大体标本、光学显微镜、扫描和透射电镜、放射身显影、微量元素和柱层析氨基酸定量测定、一氧化氮含量测定等多种观察方法进行评价。结果 新鲜和冻存的筋膜软骨细胞移植在结构、形成新的软骨细胞能力、代谢活性方面均优于游离软骨细胞移植

Repair of articular cartilage defect with a large area: an experimental study

OBJECTIVE: To compare and evaluate potentialities and biological characteristics of grafts of chondrocytes cultured on fascia, periosteum and articular cartilage in repairing large defects of articular cartilage. METHOD: The large defects of articular cartilage were repaired with grafts of freeze-stored and fresh chondrocytes cultured on fascia, periosteum and articular cartilage, which were evaluated by a number of observation methods. RESULT: The fresh and freeze-stored fascial chondrocytes were superior to free grafts of chondrocytes in potentiality of formation of new cartilaginous tissue, structure and metabolism of newly formed tissue. Relatively good results were also obtained similarly by using fresh autografts of periosteum and cartilage. CONCLUSION: Chondrocytes can be cultured normally on fascia, which can be regarded as an ideal carrier of chondrocytes.