| 乙酰葛根素对氧糖剥离诱导海马神经元凋亡的影响 |
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| 引用本文： | 刘东梅,张岫美,娄凤兰. 乙酰葛根素对氧糖剥离诱导海马神经元凋亡的影响[J]. 中国药理学通报, 2013, 29(7): 981-984 |
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| 作者姓名： | 刘东梅 张岫美 娄凤兰 |
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| 作者单位： | 1. 山东大学护理学院,山东,济南,250012
2. 山东大学医学院药理学研究所,山东,济南,250012 |
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| 基金项目： | 山东省自然科学基金资助项目(项目编号：R2010HM132) |
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| 摘 要： | 目的研究新型异黄酮类化合物乙酰葛根素对氧糖剥离神经元的保护作用及其机制。方法采用氧糖剥离建立细胞模型。DAPI法观察神经元凋亡率,RT-PCR法检测NF-κBp65、HIF-1α及p53mRNA的表达,Western blot法测定Hsp70蛋白的含量及乙酰葛根素对其的影响。结果与模型组相比较,乙酰葛根素可以减少凋亡细胞数;降低NF-κBp65、HIF-1α及p53mRNA表达;乙酰葛根素(低,中和高浓度组)增加Hsp70蛋白的表达。结论乙酰葛根素降低NF-κBp65、HIF-1α、p53的表达和升高Hsp70蛋白表达。
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| 关 键 词： | OGD/R-24h 乙酰葛根素 凋亡 NF-κBp65 HIF-1α p53 Hsp70 |
Effect of acetylpuerarin on apoptosis induced by oxygen-glucose deprivation/reperfusion in hippocampal neurons |
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| LIU Dong-mei , ZHANG Xiu-mei , LOU Feng-lan. Effect of acetylpuerarin on apoptosis induced by oxygen-glucose deprivation/reperfusion in hippocampal neurons[J]. Chinese Pharmacological Bulletin, 2013, 29(7): 981-984 |
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| Authors: | LIU Dong-mei ZHANG Xiu-mei LOU Feng-lan |
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| Abstract: | Aim To study the protection of a new isoflavone compounds-acetylpuerarin on the oxygen-glucose deprivation/reperfusion(OGD/R) damage and its mechanism.Methods Neurons were cultured to established OGD/R;4′,6-diamidino-2-phenylindole(DAPI) was used to analyze the expression of apoptotic cells;NF-κBp65,hypoxia-inducible factor-1α(HIF-1α) and p53mRNA were explored by RT-PCR;the expression of heat shock protein 70(Hsp70) protein and the effect of acetylpuerarin were determined with Western blot.Results Compare to model,acetylpuerarin could attenuate neuronal apoptosis,decrease the levels of NF-κBp65,HIF-1α and p53mRNA,and increase the expression of Hsp70 protein.Conclusion Acetylpuerarin decreases the expression of NF-κBp65,HIF-1α and p53mRNA,and increases the levels of Hsp70 protein. |
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| Keywords: | OGD/R-24h acetylpuerarin apoptosis NF-κBp65 HIF-1α p53 Hsp70 |
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