鸡Ⅱ型胶原对小鼠胶原性关节炎的治疗作用

目的　研究鸡Ⅱ型胶原 (CⅡ )对小鼠胶原性关节炎(CIA)的治疗作用。方法　在建立小鼠CIA模型的基础上 ,观测关节积分变化 ,同时检测脾淋巴细胞增殖反应 ,白细胞介素 1(IL 1)和IL 2。结果　CⅡ 5、10、2 0 μg·kg-1ig× 7d能明显减轻CIA小鼠的关节炎症 ;抑制CIA小鼠过高的ConA诱导的脾细胞增殖反应及小鼠腹腔巨噬细胞IL 1的过度产生 ,降低脾细胞IL 2的产生 ;同时病理检查亦发现CⅡ治疗用药可以减轻CIA小鼠的骨膜增生和炎细胞浸润。结论　CⅡ对CIA具有治疗作用 ,其机制与免疫功能调节有关。

Effect of chicken collagen type Ⅱ on collagen -induced arthritis in mice

AIM To investigate the effect of chicken collagen type Ⅱ (CⅡ) on collagen-induced arthritis (CIA) mice. METHODS The model of mice CIA was prepared and the change of the mean of arthritis scores was observed. Meanwhile, ConA and LPS-induced splenocytes proliferation and the production of interleukin (IL)-1 and IL-2 were measured. RESULT CⅡ [5, 10, 20 μg·kg -1 ig×7 d] significantly decreased the mean of arthritis scores, inhibited the increased ConA-induced splenocytes proliferation as well as the elevated production of IL-1 and IL-2. Meanwhile, the pathological examination showed that articulus cartilage degeneration with synovial hyperplasia and inflammatory cells infiltration in CIA mice was suppressed by ig CⅡ. CONCLUSION CⅡ has therapeutical effect on mice CIA which may be related to its immunoregulative function.