醋酸铅对胸腺细胞凋亡及p53基因表达的影响

目的探讨醋酸铅诱导小鼠胸腺细胞凋亡及对p53基因表达的影响。方法以3种不同浓度的醋酸铅灌胃饲养小鼠46d后，取胸腺组织，用琼脂糖凝胶电泳法检测胸腺细胞凋亡情况，双抗体夹心ELISA检测胸腺组织中p53的表达情况。结果铅处理组小鼠胸腺细胞有被降解的凋亡带，并且降解条带的量随着染铅剂量的增加而增加；小鼠胸腺细胞p53表达量均高于对照组，并且表达量随着染铅剂量增加而升高。结论醋酸铅可能诱发小鼠胸腺细胞凋亡；醋酸铅可以促进胸腺细胞中p53基因的表达，提示p53可能作为调控因子通过调节Bcl-2和Bax基因的表达参与铅对胸腺损害的毒性过程，高表达的p53启动凋亡过程，诱导细胞凋亡。

The Effects of Acetic Acid Lead on the Apoptosis and the Genetic Expression of p53 of Thymus Cell in Mice

Objective To investigate the influences of acetic acid lead on the apoptosis and the genetic expression of thymus cell in Mice. Methods By lavage after using the distilled water dilution acetic acid lead, took the thymus after 46 days, to detect the apoptosis by agarose gel electrophoresis and detect the genetic expression of p53 by EI.ISA. Results The thymus cell of the group that dealt with lead had a degraded apoptosis band, the quantity of which increased with the dyed lead dose. The thymus cell p53 expression dose which increased with the dyed lead dose is higher than the control group. Conclusions The acetic acid lead probably induces the apoptosis of the thymus cell in mice. The acetic acid lead promotes the p53 genetic expression of the thymus cell. This phenomenon hinted that p53 was probably the regulating factor participate in the toxic process of the thymus impaired by the lead, the apoptosis process switched on the high expression of p53, inducing the cell apoptosis by regulating the genetic expression of Bcl-2 and Bax.