姜黄素通过调控Notch通路促进大鼠脑缺血后神经干细胞增殖和迁移

 目的： 观察姜黄素对大鼠局灶性脑缺血后室管膜下区（SVZ）神经干细胞(NSCs)增殖和迁移的影响，及其与Notch信号通路的相关性。方法： 采用线栓法制备大鼠大脑中动脉缺血再灌注损伤模型，随机分成假手术组（sham）、缺血再灌注组（I/R）及姜黄素治疗组(I/R+curcumin)。动物在模型成功后1 h连续腹腔注射姜黄素7 d后处死。免疫荧光法检测各组梗死侧SVZ BrdU及BrdU/DCX标记NSCs的数目及迁移趋势。Western blotting方法检测各组Notch通路的中间产物Notch 细胞内域（NICD)的表达。结果： I/R+curcumin组的大鼠梗死侧SVZ BrdU及BrdU/DCX标记的NSC较I/R组显著增多（P＜0.05），同时其较I/R组更广泛地分布在往病灶迁移的路径上。I/R+curcumin组较I/R组的NICD生成也增多（P＜0.05）。结论： 姜黄素能促进缺血性大鼠脑SVZ NSCs增殖和迁移，其机制可能是通过激活Notch信号通路产生的。

Curcumin can Promote Proliferation and Migration of Neural Stem Cells on Cerebral Ischemia Rat maybe by Regulating Notch Signaling

Abstract Aim : To investigate the proliferation and migration of neural stem cell (NSC) in subventricular zone (SVZ) on focal cerebral ischemia with curcumin, and to explore the relationship between these effect and Notch signaling. Methods: The models were established by an intraluminal suture method to induce middle cerebral artery occlusion.in rats. Animals were randomly divided into sham group, vehicle group, and curcumin group. Animals were admined curcumin for 7days i.p. 1 hours after models succeeded, and they were sacrificed. Immunofluorescence was used to label NSC expressed by BrdU and BrdU/DCX. Western blot was used to detect Notch Intracellular Domain(NICD). Results: As compared with vehicle group, Brdu-positive and Brdu /DCX double-labeled positive cells in curcumin group were conspicuously higher than vehicle group (P＜0.05). Westernblot result showed that the lever of expression of NICD in curcumin group's was significantly higher than that of vehile group(P＜0.05) .Conclusion: Curcumin could promote NSC proliferation and migration in SVZ after focal cerebral ischemia. The possible mechanism may be that curcumin could active Notch signaling.