| 乙型肝炎病毒核心基因转基因小鼠的建立 |
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| 引用本文： | 刘红,张钦宪,胡以平. 乙型肝炎病毒核心基因转基因小鼠的建立[J]. 郑州大学学报(医学版), 2004, 39(3): 398-400 |
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| 作者姓名： | 刘红 张钦宪 胡以平 |
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| 作者单位： | 郑州大学基础医学院组织学与胚胎学教研室,郑州,450052;第二军医大学细胞生物学教研室,上海,200433 |
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| 基金项目： | 河南省重大科技攻关基金资助项目 0222031300;河南省医学创新人才工程基金资助项目 200084 |
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| 摘 要： | 目的：建立乙型肝炎病毒核心基因转基因小鼠。方法：构建乙型肝炎病毒核心基因真核表达载体pcDNA3-HBc；采用显微注射法将线性化的表达载体注射至小鼠受精卵的雄性原核巾，然后将注射存活的受精卵植入假孕母鼠的输卵管；取其产10d龄子代鼠进行鉴定。结果与结论：表达载体内切酶酶切及序列分析结果与预期一致；PCR鉴定转基因阳性小鼠比例为25％(6／24)。可表达核心蛋白基因的转基因小鼠成功建立。
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| 关 键 词： | 乙型肝炎病毒核心基因 真核表达载体 转基因动物 小鼠 |
| 修稿时间： | 2003-12-30 |
Establishment of transgenic mice for hepatitis B virus core gene expression |
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| LIU HONG ),ZHANG Qinxian ),HU Yiping ) ). Establishment of transgenic mice for hepatitis B virus core gene expression[J]. Journal of Zhengzhou University: Med Sci, 2004, 39(3): 398-400 |
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| Authors: | LIU HONG ) ZHANG Qinxian ) HU Yiping ) ) |
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| Affiliation: | LIU HONG 1),ZHANG Qinxian 1),HU Yiping 2) 1) Department of Histology & Embryology,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450052 2)Department of Cell Biology,the Second Military Medical University,Shanghai 200433 |
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| Abstract: | Aim: To establish transgenic mice for hepatitis B virus core gene expression. Methods: The HBV core gene eukaryotic expression vector was constructed and the linerized DNA was microinjected into the male pronuclei of C57BL/6 mouse fertilized eggs. The eggs survived from microinjection were transplanted into oviducts of pseudopregnant recipients. The transgenic mice were identified by PCR. Results and Conclusion: The map of endonuclease digestion and sequencing of the eukaryotic expression vector was consistent with what in anticipation. The transgene positive mice accounted for 25%(6/24). The transgenic mice for hepatitis B virus core gene expression are established successfully. |
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| Keywords: | hepatitis B virus core gene eukaryotic expression vector transgenic animal mouse |
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