| 胶质细胞源性神经营养因子对周围神经再生的作用 |
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| 引用本文： | 陈哲宇,李建红,郑兴东,路长林,何成. 胶质细胞源性神经营养因子对周围神经再生的作用[J]. 实用手外科杂志, 2001, 15(1): 26-28 |
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| 作者姓名： | 陈哲宇 李建红 郑兴东 路长林 何成 |
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| 作者单位： | 上海第二军医大学神经生物学教研室, |
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| 基金项目： | 本课题受国家重点基础研究基金资助(国家"973"课题) |
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| 摘 要： | 目的研究胶质细胞源性神经营养因子(glial cell line-derived neurotrophic factor,GDNF)对周围神经再生的作用.方法硅管套接大鼠坐骨神经,术中一次性将GDNF、生理盐水(nor-mal saline solution,SAL)分别加入硅管中.术后4周,应用溃变神经纤维染色法、辣根过氧化物酶(horseradish peroxidase,HRP)逆行追踪技术观察GDNF对轴突再生的影响.结果与SAL组相比,GDNF组渍变神经纤维面积明显减少,溃变神经纤维面积百分率由17.3%降低到1.9%(P＜0.01);GDNF组脊髓标记胞体显著增加,HRP标记胞体数的百分率由43.5%上升到68.3%(P＜0.01).结论外源性GDNF能明显促进周围神经再生.
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| 关 键 词： | 胶质细胞源性神经营养因子 坐骨神经 神经再生 |
| 修稿时间： | 2000-08-14 |
Effect of GDNF on peripheral nerve regeneration in rat |
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| CHEN Zheyu,LI Jianhong,ZHENG Xingdong,et al.. Effect of GDNF on peripheral nerve regeneration in rat[J]. Chinese Journal of Practical Hand Surgery, 2001, 15(1): 26-28 |
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| Authors: | CHEN Zheyu LI Jianhong ZHENG Xingdong et al. |
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| Affiliation: | CHEN Zheyu,LI Jianhong,ZHENG Xingdong,et al. Department of Neurobiology,The Second Military Medical University,Shanghai 200433 |
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| Abstract: | Objective To study the effect of GDNF on peripheral nerve regeneration. Methods Transectioned sciatic nerve in rat was sutured into silicone chamber. GDNF or SAL solution was injected into the silicone chambers during operation. Four weeks later, the effect of GDNF on axonal regeneration was evaluated with use of degenerative neurofiber staining and HRP retrograde tracing. Results Compared with SAL group, the percentage of degenerative neurofiber areas was decreased from 17.3% to 1.9%(P <0.01), and the percentage of labeled spinal intact neuron number was significantly increased from 43.5% to 68.3%(P < 0.01) in GDNF group. Conclusion The results suggested that exogenous GDNF could obviously enhance peripheral nerve regeneration. |
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| Keywords: | Glial cell line-derived neurotrophic factor Sciatic nerve Nerve regeneration |
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