慢性脑缺血大鼠脑OX1R表达的变化

目的：研究慢性脑缺血时脑食欲素受体-1（OX1R）的表达及其随缺血时间的变化。方法：通过结扎双侧颈总动脉建立慢性脑缺血模型，通过水迷宫对慢性脑缺血大鼠的行为学进行评价，免疫组化法观察OX1R的表达，双标免疫荧光进一步确定OX1R表达的定位。结果：缺血15d时大鼠的学习记忆能力明显减退，1月、2月较缺血15 d模型组的学习记忆能力有所好转。同时，从缺血急性期一直持续到15 d OX1R的表达明显增高，1月时OX1R的表达明显低于15d，2月时OX1R的表达再次增高。从组织学看：15d时部分细胞萎缩，1月时大部分细胞变形萎缩，2月时部分细胞形态恢复正常。双标免疫荧光证实OX1R确实在神经元有表达。结论：慢性缺血性脑损伤时OX1R的表达呈双相性变化,食欲素系统可能在缺血性脑损伤与修复过程中发挥一定的调节作用。

OX1R expression in chronic ischemic brain tissue of rats

AIM: To study the orexin-1 receptor (OX1R) expression in chronic ischemic brain tissue of rats and the change following the ischemic process. METHODS: The cerebral ischemic model was established by ligating double carotid arteries in rats. The behavior of the models was evaluated by water maze. The OX1R expression was determined by immunohistochemical technique, and the location of OX1R expression was further confirmed by double immunofluorescent staining. RESULTS: The intelligence of rats 15 d after ligating double carotid arteries was impaired obviously, and improved in 1 month and 2 months compared with the model in 15 d. At the same time, the OX1R expression increased obviously from acute phase until 15 d of cerebral ischemia and decreased notably in 1 month compared to the model in 15 d, and then increased significantly for the second time in 2 months of model. From the histology, partial neurons of 15 d model rats got atrophy, and most neurons in 1 month model rats got cytomorphosis and atrophy, however partial neurons in 2-month model rats recovered normally. The OX1R expression was confirmed in neurons definitely by double immunofluorescent staining. CONCLUSION: During the pathologic process of chronic ischemic injury, orexin system has two-way regulatory functions through.