Math1基因内耳导入后噪声性聋豚鼠听功能改变观察

目的观察Math1基因内耳导入对噪声性聋豚鼠听功能的影响，探讨Math1基因过表达对噪声损伤耳蜗的生物学效应，为内耳基因治疗提供实验基础和理论依据。方法经脉冲噪声致聋的豚鼠45只（各频率ABR阈值均≥95dB SPL）,雌雄不限，实验开始时体草250～300g。随机分为3组：Ad—Math1-EGFP组（30只）；Ad—EGFP组（5只）；空白组（10只）。各组豚鼠在基因转导后4周、8周分别测试双耳ABR。测试完毕后处死动物，观察听泡及耳蜗尤炎性病变者记录听阈结果。结果Math1导入后4周，导入耳各频率ABR阈值低于对照耳（右耳），也低于Ad—EGFP组及空内组，平均达到85dBSPL。Math1导入后8周，导入耳各频率ABR阂值低于对照耳（右耳），也低于Ad—EGFP组及空白组，与4周时比较，进一步好转，平均达到75dB SPL。结论Math1基因内耳导入可使噪声导致全聋的豚鼠听功能部分恢复，为噪声性聋的治疗打开了新的思路和手段。

Hearing improvement of NIHL guinea pig by overexpressed Math1 gene in the cochlea

Objective To evaluate the effect of adenovirus mediated Mathl gene transferring into the cochlea of noise-induced hearing loss (NIHL) guinea pig to assess its influence on hearing and to provide experiment foundation for inner ear gene therapy.Methods Forty-five NIHL guinea pigs (audibility threshold≥95 dB SPL),with no sex limited,weight between 250 and 300 g before this experiment,were divided into three groups randomly.Thirty guinea pigs (AdMath1-EGFP group) were implanted with adenovirus mediated Hath1 gene.Five guinea pigs (Ad-EGFP group) were implanted with enhanced green fluorescent protein (EGFP) and ten without treatment as blank group.Auditory brainstem response (ABR) thresholds were determined in all animals at 4 weeks and 8 weeks after surgery.Results The ABR threshold of the ear inoculated in the Ad-Math1-EGFP group was lower than the control ear (right ear) and the Ad-EGFP group and the blank group 4 weeks after surgery (85 dB SPL in average).The ABR threshold of the ear inoculated in the Ad-Math1-EGFP group was lower than the control ear (right ear) and the Ad-EGFP group and the blank group 8 weeks after surgery,and better than that 4 weeks before (75 dB SPL in average).Conclusion Math1 gene transferred into the cochlea can partially recover the hearing loss which provides a new method for NIHL therapy.