Rho-GDI2在胰腺癌中的表达及其临床意义

目的探讨鸟嘌呤核苷酸解离抑制因子2（Rho—GDI2）基因在胰腺癌组织中的表达及其临床意义。方法运用RT-PCR技术和免疫组化技术检测60例胰腺癌组织及配对的癌旁组织中Rho—GDI2mRNA和蛋白的表达，分析胰腺癌Rho-GDI2的表达与临床病理参数间的关系。结果胰腺癌组织及相应癌旁组织中Rho-GDI2mRNA表达量分别为0．661±0．021和0．199±0．023；Rho—GDI2蛋白阳性表达率分别为73．3％（44／60）和41．7％（25／60），癌组织的表达均显著高于癌旁组织，差异有统计学意义（P值均〈0．01）。胰腺癌组织Rho-GDI2蛋白表达与患者性别、年龄及肿瘤部位无相关性，而与肿瘤大小、分化程度、病理分期、淋巴结转移、血管侵犯等相关（P值均〈0．05）。结论Rho-GDI2在胰腺癌组织中过表达，且与胰腺癌的恶性生物学行为密切相关。

Expression and clinical significance of Rho GDP dissociation inhibitor 2 in pancreatic cancer

Objective To investigate the expression and clinical significance of Rho GDP dissociation inhibitor 2 （Rho-GDI2） in pancreatic cancer. Methods Immunohistochemistry and RT-PCR were used to evaluate the expression of Rho-GDI2 in 60 pairs of pancreatic cancer tissues and adjacent pancreatic tissues, and its correlation with clinicopathological parameter of pancreatic cancer was also analyzed. Results The expressions of Rho-GDI2 mRNA were 0. 661 ± 0. 021 and 0. 199 ± 0.023 in pancreatic cancer tissues and adjacent pancreatic tissues, and the positive rate of Rho-GDI2 protein expression were 73.3% （44/60） and 41.7% （25/60） , the positive rate of Rho-GDI2 expression in pancreatic cancer tissues was higher than that of adjacent normal tissues, and the difference between the two groups was statistically significant （P 〈0.01 ）. The expression of Rho-GDI2 was strongly correlated with tumor size, differentiation, staging, lymph node metastasis, vascular invasion （P〈0.05）, but it was not associated with gender, age, tumor location （P 〉0.05）. Conclusions Rho-GDI2 expression is up-regulated in pancreatic cancer, and is strongly correlated with the malignant biological behavior of pancreatic cancer.