In Vitro Antiplatelet Activity of Flavonoids from Leuzea Carthamoides |
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Authors: | Vit Koleckar Eliska Brojerova Zuzana Rehakova Katerina Kubikova Frantisek Cervenka Kamil Kuca |
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Institution: | 1. Department of Pharmaceutical Botany and Ecology, Faculty of Pharmacy, Charles University, Hradec Kralove, Czech Republic;2. Center of Advanced Studies, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic;3. Center of Advanced Studies, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic;4. Department of Toxicology, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic;5. Department of Chemistry, Faculty of Sciences, J.E. Purkinje University, Usti nad Labem, Czech Republic |
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Abstract: | Plants and their secondary metabolites, including flavonoids, exhibit a wide range of biological effects. Consequently, natural substances are receiving an increased attention in medicinal research. Owing to these facts, in vitro antiplatelet activity of ethanol summary extract and four flavonoids from Leuzea carthamoides was determined in human platelet-rich plasma. Arachidonic acid (AA), adenosine diphosphate (ADP), collagen (COL), and thrombin were used as agonists of platelet aggregation. The summary extract showed a significant inhibition of the aggregation induced by COL and ADP. Of the tested flavonoids, eriodictyol (1) and patuletin (2) influenced COL- and AA-induced aggregation. Their IC50 values are presented. Flavonoid glycosides eriodictyol-7-β-glucopyranoside (3) and 6-hydroxykaempferol-7-O-(6″-O-acetyl-β-Dsmall cap]-glucopyranoside) (4) were found to be weak antiplatelet agents. These results confirmed the fact that glucosylation decreases the antiplatelet activity. Quantitative composition of tested flavonoids in L. carthamoides extract was also determined. Though two of the tested flavonoids inhibited platelet aggregation, further evaluation of L. carthamoides, in order to discover other antiplatelet active compounds and possible adverse health effects, is needed. |
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Keywords: | Antiplatelet activity Flavonoids Leuzea carthamoides Aggregation Plants Quantity |
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