干式化学法检测健康儿童青少年血清电解质和空腹血糖参考区间与相应的传统参考区间差别不大

目的 建立深圳地区3月龄至18周岁健康人群干式化学法血清K+、Na+、Cl-、总钙（TCa）、总二氧化碳（TCO2）、空腹血糖（FBG）的参考区间值。方法 根据儿童青少年生长发育规律分为3月龄至3岁、～6岁、～12岁和～18岁组。标本来源为健康体检常规检测后的剩余标本。采用美国强生公司VITROS 350干式化学分析仪检测血清K+、Na+、Cl-、TCa、TCO2和FBG水平，比较不同年龄组和不同性别间的差异，并确定6项检测指标在3月龄至18岁健康人群中的参考区间值。结果 1 898名儿童青少年合格标本进入分析，其中男1 033名,女865名。3月龄至3岁组163名，～6岁组791名，～12岁组613名，～18岁组331名。①各年龄组男童TCa检测结果均低于女童，～6岁组男童FBG检测结果高于女童，差异均有统计学意义（P均<0.05），其余各组不同性别间差异均无统计学意义（P均﹥0.05）。Na+、Cl-、TCa和FBG在同性别不同年龄组间差异均有统计学意义（P均<0.05），K+和TCO2在同性别不同年龄组间差异均无统计学意义（P均﹥0.05）。②K+、Na+、Cl-、TCa、TCO2和FBG在3月龄至18岁健康人群中的参考区间值（P2.5～P97.5）分别为3.4～4.9、134～147、98～110、2.18～2.60、19～28和3.6～6.0 mmol·L-1。③空腹血糖受损（IFG）总检出率为2.3%（男2.9%，女1.6%）。3月龄至3岁组未检出IFG，～6岁、～12岁和～18岁组IFG检出率分别为1.0%、3.4%和4.5%。各年龄组间IFG检出率差异均有统计学意义（P均﹤0.05），同一年龄组间男童IFG检出率均高于女童（P均<0.05）。结论 以深圳地区3月龄至18岁健康儿童为人群的干式化学法血清K+、Na+、Cl-、TCa、TCO2和FBG的参考区间值在不同性别和年龄总体差别不大，与既往国内外相关研究差别不大。

There is no significant difference in the reference intervals of children and adolescents serum electrolyte and fasting blood-glucose between dry chemistry analyzer and traditional methods

Objective To investigate the reference intervals of serum kalium ion (K+), natrium ion (Na+), chlorine ion (Cl-), total calcium (TCa), total carbon dioxide (TCO2) and fasting blood glucose (FBG) among healthy children and adolescents(aged from 3 months to 18 years) by dry chemical system in Shenzhen.Methods Healthy children and adolescents were divided into four groups by age according to the rule of growth and development: 3 months to 3 years, -6 years, -12 years and -18 years age groups. The serum was from the remaining specimens of routine physical examination after routine testing. Serum K+, Na+, Cl-, TCa, TCO2 and FBG were detected by the VITROS 350 system, and the differences between different age groups and different genders were compared and the reference intervals of 6 items among healthy children and adolescents aged from 3 months to 18 years were established.Results A total of 1 898 qualified specimens from children and adolescents were recruited, including 1 033 males and 865 females, with 163, 791, 613 and 331 subjects for 3 months to 3 years group, -6 years group, -12 years group and -18 years group. ① TCa in boys was significantly lower than that in girls, but FBG of -6 years group in boys was significantly higher than that in girls, there was no significant difference between genders in other groups (P>0.05). Na+, Cl-,TCa and FBG significantly varied among age groups in each gender (P<0.05), however K+and TCO2 did not (P>0.05). ②Reference intervals of serum K+, Na+, Cl-, TCa, TCO2 and FBG were 3.4-4.9, 134-147, 98-110, 2.18-2.60, 19-28, 3.6-6.0 mmol·L-1 respectively. ③The overall detection rate of IFG was 2.3% (2.9% for male and 1.6% for female).There was no detectale IFG in 3 months to 3 years group,the detection rate of IFG in -6 years, -12 years, -18 years groups was 1.0%, 3.4% and 4.5%, respectively, varying significantly among age groups (P<0.05), being more common in boys than that in girls (P<0.05).Conclusion Reference intervals of serum K+, Na+, Cl-, TCa, TCO2 and FBG are established by dry chemical system among healthy children and adolescents in Shenzhen. It has little difference in different ages, genders and previous studies of domestic and foreign.