白花蛇舌草对人胶质瘤细胞增殖及凋亡的影响及其机制

目的 观察白花蛇舌草在体外对胶质瘤U87细胞株增殖和凋亡的影响并探讨其机制.方法 用不同浓度的白花蛇舌草含药培养基(0、5、10、15、20ml/L)处理U87细胞不同时间(24、72 h)后,采用噻唑蓝(MTT)比色法检测其对U87细胞增殖能力的影响,流式细胞仪检测对照组与实验组细胞的凋亡差异,免疫印迹法检测U87细胞中半胱氨酸蛋白酶(Caspase-3)表达水平的改变.结果 经白花蛇舌草5、10、15、20ml/L处理后,U87细胞增殖受到明显抑制,呈显著的剂量依赖性;经白花蛇舌草(5、10 ml/L)处理后U87细胞的凋亡率分别增加到(5.22±0.29)%、(8.38±0.65)%,明显高于对照组的(3.09±0.05)%(P＜0.01);白花蛇舌草可明显上调U87细胞中Caspase-3的蛋白表达,且呈浓度依赖性.讨论白花蛇舌草对胶质瘤U87细胞生长的抑制作用可能通过上调Caspase-3基因的表达从而诱导细胞凋亡实现.

Effects of hedyotic diffusa on human glioma cells proliferation and apoptosis and the mechanism

Objective To investigate the effects of hedyotic diffusa (HD) on glioma U87 cells proliferation and apoptosis and the possible mechanism. Methods U87 cells were cultured for 24 and 72 h in medium containing HI) with different concentrations (0, 5, 10, 15, 20 ml/L). The methylthiazol tetrazolium (MTT) assay was used to detect the survival rate, the apoptosis of the cells was examined by flow cytometry, and the change of caspase-3 protein level was measured by Western blotting. Results The cells proliferation was obviously inhibited by HD in a dose-dependent manner. The apoptosis rate in 5, 10 ml/L HD groups was (5.22 ± 0. 29) % and ( 8. 38 ± 0.65 ) % respectively, which was significantly higher than that in 0 ml/L HD group (P ＜ 0. 01 ). The protein expression of caspase-3 was increased dose-dependently. Conclusion The growth inhibition of glioma U87 cells caused by HD may be associated with the upregulation of caspase-3 gene, which induced apoptosis.