比较基因组杂交检测散发性结直肠癌染色体变异的临床意义

目的了解散发性结直肠癌（SCRC）的染色体变异及其与SCRC临床病理特征的关系。方法采用比较基因组杂交（CGH）技术检测40例SCRC的染色体变异情况，分析其与临床病理特征的关系。结果40例SCRC患者CGH检测结果显示．所有病例均有不同程度的染色体臂发生扩增或丢失，平均每例变异数为7．55，扩增数为4．73，丢失数为2．83。染色体扩增区域有20q、12q、13q、7P、7q和16q；缺失区域有18q、5q、4q、8P和17P。结直肠癌TNM分期中Ⅲ、Ⅳ期患者的染色体总变异数和扩增数、缺失数均高于Ⅰ、Ⅱ期患者。本组患者染色体总变异数、扩增数和丢失数在不同的肿瘤部位、组织学类型和分化程度间比较．差异均无统计学意义。20q的扩增与TNM分期有关。结论染色体变异在SCRC中普遍存在，SCRC的染色体变异数及20q的扩增与TNM分期有关。

Detection of choromosomal aberration in sporadic colorectal cancer with comparative genomic hybridization

Objective To investigate the choromosomal aberration in sporadic colorectal carcinoma and its association with clinieopathologieal features. Methods Comparative genomic hybridization(CGH) was used to screen the changes in the number of DNA sequence copies in 40 sporadic coloreetal cancer patients in order to identify regions that contain genes important for the development and progression of colorectal cancer. Results In 40 sporadic colorectal cancer, frequent gain at 20 q, 12 q, 13 q, 7 p, 7 q and 16 q were found, while loss was also found at 18 q, 5 q, 4 q, 8 pand 17 p. The number of choromosomal aberration was closely associated with tumor stage (P<0.05).No significant association was found between the number of choromosomal aberration and tumor site, histopathologic type and histologic grade. Conclusions Choromosomal aberration exists generally in sporadic colorectal carcinoma. The number of choremosomal aberration and gain of 20q are closely associated with tumor stage.