原发性免疫性血小板减少症患者外周血细胞miRNA差异表达分析

本研究通过建立原发性免疫性血小板减少症(ITP)外周血细胞微小RNA(miRNA)表达谱,探讨miRNA在ITP患者和正常健康对照者外周血细胞中的表达差异。应用Exqion miRCURYTM芯片分析ITP患者和正常对照者外周血白细胞miRNA差异表达;分层聚类分析ITP患者和正常对照者之间miRNA表达谱的差异;应用实时PCR方法对表达差异miRNA进行验证。结果表明,在ITP患者与正常对照者外周血白细胞间有159个差异表达的miRNA,其中79个表达显著上调,80个表达显著下调。分层聚类分析显示,ITP存在特异性miRNA表达谱。实时PCR检测结果与芯片分析结果一致。结论:ITP患者外周血细胞存在miRNA表达异常,提示miRNA表达变化可能参与了ITP的发病。

Analysis of miRNA Differential Expression in Peripheral Blood Cells of Primary Immune Thrombocytopenia Patients

This study was purposed to clarify the difference of microRNA（miRNA） expression in the peripheral blood cells of patients with primary immune thrombocytopenia（ITP） and normal controls.Exqion miRCURYTM microarray was used to investigate differentially expressed miRNA of peripheral blood cells obtained from affected ITP patients and the healthy controls.Cluster analysis was used to identify miRNA expression profile between the ITP patients and the healthy controls.Real-time PCR was used for validation.The results showed that a total of 159 miRNA were found to be differentially expressed in ITP patients compared to the controls,with 79 up-regulated and 80 down-regulated.Based on these differentially expressed miRNA,a tree with clear distinction between the controls and ITP patients was generated by cluster analysis.Real-time PCR confirmed microarray analysis results.It is concluded that differentially expressed miRNA were found in the peripheral blood cells from ITP patients,which may be potential novel biomarkers for ITP as well as help to elucidate pathogenic mechanisms of ITP.