Th17细胞及白介素17在原发性免疫性血小板减少症中的表达和临床意义

本研究检测原发性免疫性血小板减少症(ITP)患者外周血中Th17细胞比例和IL-17水平的变化,并探讨其临床意义。应用流式细胞术和ELISA方法分别检测48例ITP患者及28例对照组外周血中Th17细胞比例及IL-17水平。结果显示,ITP患者Th17细胞比例为(1.40±1.35)%,较对照组升高(P<0.05),Th17细胞比例在糖皮质激素治疗组低于未使用糖皮质激素治疗组(P<0.05)。ITP患者Th17分泌的IL-17量为(19.624±5.187)pg/ml,较正常对照组升高(P<0.05),但IL-17水平在糖皮质激素治疗组与未使用糖皮质激素治疗组的差异无统计学意义。结论:Th17细胞在ITP发病中可能发挥一定作用,糖皮质激素可能通过抑制Th17细胞发挥治疗作用。

Expressions of Th17 Cells and Interleukin 17 in Patients with Primary Immune Thrombocytopenia and Their Clinical Significance

The aim of this study was to detect the rate of T-helper（Th） 17 cells and interleukin（IL）-17 level in peripheral blood of patients with primary immune thrombocytopenia（ITP） and to explore their clinical significance.The proportion of Th17 cells from 48 patients with ITP and 28 healthy controls was detected by flow cytometry,and the IL-17 level was evaluated by enzyme-linked immunosorbent assay（ELISA）.The results showed that the percentage of Th17 cells in ITP group was（1.40±1.35）%,which was significantly higher than that in healthy control group（P0.05）,but in the glucocorticoid hormone-treated group it was significantly lower than that in treated group without glucocorticoid hormone（P0.05）.The level of IL-17 expressed by Th17 cells in ITP patients was（19.624±5.187） pg/ml,which was higher than that in the healthy control group（P0.05）,it was lower in the glucocorticoid hormone treated group than that in treated group without glucocorticoid hormone,but there was no statistically significant difference between the glucocorticoid treated and treated group without glucocorticoid hormone（P0.05）.It is concluded that the Th17 cells may involve in the pathogenesis of ITP,and the glucocorticoid hormone probably plays a therapeutic role through inhibiting Th17 cells.