| 表没食子儿茶素没食子酸酯对人膀胱癌T24细胞增殖的影响 |
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| 引用本文： | 秦杰,郑祥毅,王云彬,白宇,谢立平.表没食子儿茶素没食子酸酯对人膀胱癌T24细胞增殖的影响[J].杭州医学高等专科学校学报,2008,28(1):1-4. |
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| 作者姓名： | 秦杰 郑祥毅 王云彬 白宇 谢立平 |
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| 作者单位： | 浙江大学医学院附属第一医院泌尿外科,浙江杭州310003 |
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| 基金项目： | 浙江省医药卫生科学研究计划 |
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| 摘 要： | 目的研究表没食子儿茶素没食子酸酯（（一）-epigallocatechin gallate，EGCG）在体外对人膀胱癌细胞系T24细胞增殖和细胞周期的影响及其可能机制。方法不同浓度的EGCG作用T24细胞24h后，应用MTT法、集落形成试验和碘化丙锭（PI）染色分别观察T24细胞在增殖、集落形成和细胞周期等方面的变化；并检测CyclinD1和CDK4的变化。结果20—100μg／mL EGCG对T24细胞作用24h后的抑制率分别为4％-61％，与对照组相比，当EGCG为40—100μg／mL时抑制率有显著性差异（P〈0．05）。EGCG能抑制T24细胞集落形成，当浓度为20和40μg／mL时能完全抑制其细胞集落形成。EGCG能使T24细胞出现GO／G1期阻滞，与对照组相比，在40-80μg／mL时有统计学意义（P〈0．05）。EGCG能抑制细胞Cyclin D1和CDK4的表达。结论EGCG能显著抑制T24细胞增殖并诱导GO／G1期阻滞，后者可能与抑制Cyclin D1和CDK4表达有关。
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| 关 键 词： | 膀胱癌 细胞周期 EGCG |
| 文章编号： | 1008-4894(2008)01-0001-04 |
| 修稿时间： | 2006年6月7日 |
Effects of EGCG on proliferation of human bladder carcinoma T24 cells |
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| QIN Jie,ZHENG Xiang-yi,WANG Yun-bin,BAI Yu,XIE Li-ping.Effects of EGCG on proliferation of human bladder carcinoma T24 cells[J].Journal of Hangzhou Medical College,2008,28(1):1-4. |
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| Authors: | QIN Jie ZHENG Xiang-yi WANG Yun-bin BAI Yu XIE Li-ping |
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| Institution: | QIN Jie,ZHENG Xiang-yi,WANG Yun-bin,BAI Yu,XIE Li-ping(Department of Urology,The First Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou,Zhejiang 310003,China) |
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| Abstract: | Objective The present study was undertaken to study the effects of(-)-epigallocatechin gallate(EGCG) on growth inhibition and cell cycle,and to investigate the corresponding molecular mechanism of T24 cells.Methods The cytotoxic,antiproliferative and cell cycle arrest effects of EGCG on T24 cells were determined with varying concentration of EGCG treatment for 24 hours by MTT assay,anchorage-dependent colony-forming assay and flow cytometry.The cell cycle modulating protein Cyclin D1 and CDK4 were determine... |
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| Keywords: | bladder cancer cell cycle EGCG |
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