放射性核素骨显像在骨缺损修复实验中的研究

目的：通过动物实验探讨多孔纳米羟基磷灰石人工骨（nano-HA）的骨缺损修复作用及放射性核素骨显像在骨重建过程中的应用，为临床运用于骨缺损修复领域提供依据。方法：采用新西兰大白兔24只在单侧桡骨制备骨缺损动物模型，然后用多孔纳米羟基磷灰石人工骨材料进行植入骨缺损处进行修复作为实验组，以普通羟基磷灰石人工骨材料作为对照组；术后2周，4周，8周和12周分别行放射性核素骨显像监测两组人工骨对骨缺损的修复能力。结果：通过ROI方法定量对比两种材料植入区和正常区放射性浓集比值，nano-HA人工骨组成骨作用优于HA人工骨组，骨缺损修复能力优于后者，差异有统计学意义（P〈0．05）。结论：纳米羟基磷灰石人工骨具有良好的成骨能力，可望成为新型的骨缺损修复材料，放射性核素骨显像在骨修复过程中具有良好的监测作用。

The experimental study on radionuclide bone imaging in repairing bone defect

Objective: To explore the ability of nano-hydroxyapatite artificial bone in repairing of large segmental bone defect and evaluate the value of radionuclide bone imaging in monitoring the bone's repairing. Methods: The animal model of bone defect was made on the bilateral radius of 24 New Zealand white rabbits,which were randomly divided into the experimental group(the defect was repaired with nano-HA artificial bone) and the control group(the defect was repaired with HA artificial bone) with 12 rabbits in each group. The ability of bone defect repair was evaluated by using radionuclide bone imaging at 2,4,8 and 12 weeks postoperatively. Results: The counts of regions of interest (ROI) measure and the target to non-target ratios(T/NT) of the two groups indicated that the experimental group stimulated more bone formation than that of the control group. The differences of the bone reconstruction ability between two groups were statistically significant (P<0.05). Conclusions: The nano-HA artificial bone improve the bone formation greatly. It can be used for the treatment of bone defects. Radionuclide imaging may be an effective and first choice for early monitoring of the bone's reconstruction.