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| 心房颤动患者血清和组织miR-223及MMP-9的表达及临床价值 | |
| 引用本文： | 袁书国,徐新,何凤屏,张社兵,范文茂.心房颤动患者血清和组织miR-223及MMP-9的表达及临床价值[J].分子诊断与治疗杂志,2014,6(5):317-324. |
| 作者姓名： | 袁书国 徐新 何凤屏 张社兵 范文茂 |
| 作者单位： | 1. 汕头大学医学院附属粤北人民医院心血管内科,广东,韶关512026;广东医学院第一临床学院,广东,湛江424000 2. 汕头大学医学院附属粤北人民医院心血管内科,广东,韶关512026 3. 汕头大学医学院附属粤北人民医院检验科,广东,韶关512026 |
| 基金项目： | 广东省自然科学基金，韶关市科技局项目 |
| 摘 要： | 目的探索miRNA-223在房颤患者心房肌组织和血清的表达差异及miRNA-223与MMP-9在房颤患者的表达水平及在房颤结构重构的发生机制。方法患有风湿性心瓣膜病接受心脏瓣膜置换术的患者31例,其中窦性心律患者15例,慢性房颤患者16例。另选取15名健康人血清作为对照组。实时荧光定量PCR检测心房肌组织和血清miRNA-223的相对表达量。ELISA及免疫组织化学检测MMP-9蛋白的表达及分布水平;HE染色及Masson染色观察心肌组织病理结构及胶原纤维含量。结果与窦性心律组相比,房颤组心房肌组织miRNA-223和血清miRNA-223表达水平均显著升高（0.0603±0.0228 vs.0.0261±0.0035,P〈0.01;4.2281±0.9165 vs 2.7613±1.2166,P〈0.05）;房颤组血清MMP-9表达水平高于窦性心律组（4.2281±0.9165 vs 2.7613±1.2166,P〈0.05）;MiRNA-223表达水平与MMP-9蛋白表达呈正相关（r=0.901,P〈0.05）。结论 MiRNA-223表达增高及正向调控MMP-9的表达,共同影响心肌胶原的代谢,促进心肌纤维化,参与房颤发生与维持。miRNA-223有望成为一种新型风湿性心脏病合并房颤的诊断标志物。 |
| 关 键 词： | 心房颤动 微小RNA 金属基质蛋白酶-9 心肌纤维化 心肌结构重构 |
The expression and clinical value of miR-223 and MMP-9 in right atrium and blood serum in patients with atrial fibrillation |
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| YUAN Shuguo,XU Xin,HE Fengping,ZHANG Shebing,FAN Wenmao.The expression and clinical value of miR-223 and MMP-9 in right atrium and blood serum in patients with atrial fibrillation[J].Journal of Molecular Diagnosis and Therapy,2014,6(5):317-324. | |
| Authors: | YUAN Shuguo XU Xin HE Fengping ZHANG Shebing FAN Wenmao |
| Institution: | YUAN Shuguo, XU Xin, HE Fengping, ZHANG Shebing, FAN Wcnmao (1. Department of Cardiology, The Yuebei Hospital Affiliated to Shantou University, Shaoguan, Guangdong, China, 512026; 2. Department of Clinical Laboratory, Yuebei Hospital Affiliated to Shantou University, Shaoguan, Guangdong, China, 512026; 3. The first clinical college of Guangdong Medical College, Zhanjiang, Guangdong, China, 524000) |
| Abstract: | Objective To investigate the change of miRNA-223 in right atrium and blood serum in patients with rheumatic heart disease and atrial fibrillation.To study the expression level of miRNA-223 and MMP-9 and possible mechanism of myocardial remodelling in patients with atrial fibrillation.Methods Right atrium appendages were obtained from 15 rheumatic valvular heart disease patients with sinus rhythm and 16 patients with chronic atrial fibrillation and another 15 healthy subjects served as a control.Expressions of miRNA-223 were detected by Quantitative real time polymemse chain reaction.MMP-9 protein expression was detected by ELISA and IHC.Collagen deposition was estimated by HE and Masson staining.Pathologic structure change of myocardial tissue in patients were observe.Results Expressions of miRNA-223 in the myocardial tissue and serum were increased（relative expression：0.0603 ± 0.00228 vs.0.0261 ±0.0035,P〈0.01;4.2281 ± 0.9165 vs.2.7613 ± 1.2166,P〈0.05） in AF patients.Expressions of MMP-9 was obvious increased（relative expression of MMP-9：4.2281 ±0.9165 vs.2.7613 ± 1.2166,P〈0.05） in AF patients.Expression of miRNA-223 was positively correlated to MMP-9（r = 0.901,P〈0.05）.Conclusion Expression of miRNA-223 level was significantly up-regulated in patients with AF,possibly contributing to up-regulation of MMP-9 leading to increase collagen content and promote myocardial fibrosis,which were involved in the occurrence and maintain of atrial fibrillation.miRNA-223 may be used as a new potential diagnostic biomarker for rheumatic heart disease complicated with atrial fibrillation. |
| Keywords: | Atrial fibrillation MicroRNA Matrix metalloproteinase-9 Myocardial fibrosis Myocardial remodelling |
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