Immunofluorescent localization ofSchistosoma mansoni circulating cathodic antigen in tissues of infected mice using monoclonal antibody |
| |
| Authors: | A. M. Deelder I. El-Dosoky E. A. E. Van Marck Z. L. Qian |
| |
| Affiliation: | (1) Laboratory of Parasitology, Medical Faculty, State University of Leiden, Wassenaarseweg 62, PO Box 9605, 2300 RC Leiden, The Netherlands;(2) Laboratory of Histopathology and Experimental Schistosomiasis, Prince Leopold Institute of Tropical Medicine, Antwerpen, Belgium;(3) Department of Parasitology, Shanghai Second Medical College, Shanghai, People's Republic of China;(4) Present address: Pathology Department Mansoura Faculty of Medicine, Mansoura, Egypt |
| |
| Abstract: | In the present study the kinetics of the uptake and deposition of the major circulating cathodic antigen (CCA) ofSchistosoma mansoni in liver, spleen, and kidney ofS. mansoni infected Swiss mice was investigated in relation to the duration of infection and infection dose (50, 100, 200 cercariae). The presence of antigen was studied with a direct immunofluorescence reaction on frozen sections of the mouse organs, using a fluorescein isothiocyanate (FITC)-labelled mouse IgM monoclonal antibody recognizing a repeating epitope of CCA.CCA was demonstrable from 2 weeks post infection (p.i.) onwards in Kupffer cells in the liver, from 3–4 weeks p.i. onwards in macrophages in the marginal zones in the spleen and from 8 weeks p.i. onwards in kidney glomeruli. The immunofluorescence reactions on CCA in kidney glomeruli, however, remained relatively weak. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
