缺氧后幼鼠大脑内巢蛋白、神经元特异性烯醇化酶的表达及其对幼鼠学习记忆能力的影响

目的:探讨缺氧对新生大鼠海马区巢蛋白、神经元特异性烯醇化酶(NSE)的表达及幼年大鼠学习记忆能力的影响.方法:将48只7d龄SD大鼠随机分为缺氧组和对照组各24只,缺氧组通过低张性缺氧建立新生鼠缺氧模型.于缺氧24h后,两组各取16只幼鼠取其大脑组织进行巢蛋白、NSE免疫组织化学显色,两组剩余8只大鼠于生后30d Morris水迷宫测试后进行大脑NSE免疫组织化学显色.结果:缺氧组幼鼠海马巢蛋白阳性细胞的积分光密度(IOD)值明显高于对照组,而缺氧组NSE阳性细胞的IOD值明显低于对照组.Morris水迷宫检测中缺氧组幼鼠在目标象限内搜寻时间缩短.结论:缺氧引起新生鼠神经干细胞增殖,却导致神经元数量减少,幼鼠的学习记忆能力明显下降.

Expression of nestin and neuron-specific enolase in hypoxic brain of rats and its effects on learning and memory

Objective: To explore the expression of nestin and neuron-specific enolase (NSE) in the brain tissue of rat pups after hypoxia and its effects on learning and memory. Methods: 7-day-old SD rats were divided into control group (CG, n=24) and hypoxia group (HG, n=24) that established by means of hypotonic hypoxia. The brain tissues of 14 rat pups from each group were detected using immunohistochemistry to explore the expression of nestin and NSE proteins 24 hours after hypoxia. The expression of NSE in remaining 16 rat pups was detected using immunohistochemistry after the water maze was performed at postnatal day 30. Results: IOD of nestin positive cells in hippocampus of HG was higher than that in CG, whereas the IOD of NSE positive cells in hippocampus of HG was lower than that in CG. The hypoxic pups spent less time in target quadrant in the Morris water maze experiment. Conclusion: Hypoxia can stimulate the neural stem cells into proliferation and the neurons into degeneration, therefore, descend the learning and memory capability.