针刺对实验性脑出血大鼠不同脑区及心肌内皮素mRNA表达的影响

目的：探讨针刺治疗脑心综合征的作用机制。方法：将实验性大鼠随机分为正常组、假手术组、脑出血组、针刺组、针刺时照组,针刺组取“内关“水沟”“人迎”,针刺时照组取“神门”“膻中”。采用组织原位杂交技术检测其中枢心血管特定调节区域及心肌内皮索（ET）mRNA表迭的动态变化,并对阳性反应物进行图像定量分析。结果：实验性脑出血可迅速诱导ET基因在脑出血周围区、下丘脑、脑干、海马、心肌的异常表达。脑出血6h即可见ETmRNA表达上调。到24h达高峰,72h时段虽略有减少,但仍高于正常水平。同时心肌的ET表达增多。针刺可阻断由脑出血诱导的ET基因表达的增加。结论：针刺通过阻断脑出血诱导的ET基因表达的增加。起到时脑出血造成的神经元及心肌损伤的保护作用。

Effects of acupuncture on ET mRNA expressions in different brain regions of the rat of experimental cerebral bleeding

OBJECTIVE: To probe into the mechanism of acupuncture for treatment of cerebro-cardiac syndrome. METHODS: The rats were randomly divided into a normal group, a sham operation group, a cerebral bleeding group, an acupuncture group and an acupuncture control group. The acupuncture group were treated with acupuncture at "Neiguan" (PC 6), "Shuigou" (GV 26), "Renying" (ST 19), and the acupuncture control group with acupuncture at "Shenmen" (HT 7) and "Danzhong" (CV 17). The tissue in situ hybridization technique was used to detect dynamic changes of endothelin (ET) mRNA expression in both the central cardiovascular special regulative area and the myocardium, and imaging quantitative analysis on the positive response materials was carried out. RESULTS: The experimental cerebral bleeding could rapidly induce abnormal expression of ET gene in the cerebral bleeding surrounding area, hypothalamus, brain stem, hippocampus and myocardium. Up-regulation of FT mRNA expression could be found at 6 h of cerebral bleeding, and it reached to the peak at 24 h, and slightly reduced at 72 h, still being higher than the normal level. At the same time, ET expression in the myocardium increased. Acupuncture could block the increase of FT gene expression induced by cerebral bleeding. CONCLUSION: Acupuncture exerts protective action on injuries of neurons and myocardium induced by cerebral bleeding via blocking the increase of FT gene expression induced by cerebral bleeding.