| 还原型谷胱甘肽对实验性视神经炎视神经和视网膜的影响 |
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| 引用本文： | 陆孟婷,李平华.还原型谷胱甘肽对实验性视神经炎视神经和视网膜的影响[J].第三军医大学学报,2009,31(7). |
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| 作者姓名： | 陆孟婷 李平华 |
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| 作者单位： | 重庆医科大学附属第一医院眼科,重庆,400016 |
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| 摘 要： | 目的 观察还原型谷胱甘肽(GSH)对实验性视神经炎视神经和视网膜的影响,并探讨其作用机制.方法 以48只雌性SD大鼠建立实验性变态反应性脑脊髓炎(EAE)动物模型,完全随机分为EAE组18只和GSH组30只,另6只雌性SD大鼠为正常组.免疫后当天开始,GSH组给予注射用GSH 100 mg·kg-1·d-1腹腔注射14 d,EAE组给予等量生理盐水替代.分别于发病初发期、高峰期、恢复期观察视神经病理学改变、视网膜神经节细胞(RGCs)凋亡,并检测视网膜丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-PX)、一氧化氮(NO)的含量.结果 GSH组18只大鼠发病,发病率为60%,症状评分为1～3分;视神经HE染色见神经纤维排列较规则,胶质细胞较EAE组有所增加,脱髓鞘相对轻;RGCs凋亡数初发期和高峰期比EAE组减少,差异有统计学意义(P<0.01);视网膜MDA、NO含量下降,GSH-PX活力升高,与EAE组比较差异有统计学意义(P<0.05).结论 还原型谷胱什肽可降低EAE大鼠发病率和症状评分,抑制大鼠RGCs的凋亡,降低视网膜MDA、NO含量,提高GSH-PX活力,对实验性视神经炎的视神经和视网膜具有保护作用.
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| 关 键 词： | 实验性变态反应性脑脊髓炎 视神经 凋亡 还原型谷胱甘肽 |
Reduced glutathione protects retina and optic nerve of rats after optic neuritis |
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| LU Meng-ting,LI Ping-hua.Reduced glutathione protects retina and optic nerve of rats after optic neuritis[J].Acta Academiae Medicinae Militaris Tertiae,2009,31(7). |
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| Authors: | LU Meng-ting LI Ping-hua |
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| Institution: | LU Meng-ting,LI Ping-hua(Department of Ophthalmology,First Affiliated Hospital,Chongqing Medical University,Chongqing 400016,China) |
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| Abstract: | Objective To investigate the effect of reduced glutathione(rGSH)on the retina and optic nerve in rats with experimental optic neuritis,and the underlying mechanism.Methods Animal model of experimental allergic encephalomyelitis(EAE),which was as used for acute demyelinating optic neuritis were established in 48 female SD rats,and then the rats were randomly divided into EAE group(n=18)and GSH group(n=30).Another 6 female SD rats served as normal control.Rats of GSH group received an intraperitoneal injectio... |
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| Keywords: | RGCs MDA GSH-PX NO |
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