大鼠牙囊细胞集落刺激因子1受体的研究

目的 研究不同浓度集落刺激因子1(colony—stimulating factor-1，CSF-1)、白细胞介素1α(interleukin—1α，IL—1α)对大鼠牙囊细胞CSF—1受体基因表达的影响；明确CSF—1自分泌作用的抑制机制，为研究细胞因子在牙齿萌出和牙槽骨吸收中的作用奠定基础。方法 间接免疫酶标法检测体外培养牙囊细胞，以及出生后不同时期牙囊组织中CSF—1受体蛋白表达。逆转录多聚酶链反应法(RT—PCR)检测在细胞培养液中分别加入不同浓度CSF—1、IL—1α对牙囊细胞CSF—1受体基因表达的影响。结果 间接免疫酶标法染色显示CSF—1受体蛋白在生后早期牙囊中染色明显，10d后染色不明显或者完全无染色，高浓度CSF—1作用下，牙囊细胞CSF—1受体mRNA表达明显消失。不同浓度IL—1α作用后，CSF—1受体mRNA水平保持不变。结论 CSF—1受体蛋白在出生后早期牙囊中表达明显，随后逐渐消失。高浓度CSF—1抑制CSF—1受体基因的表达，不同浓度IL—1α对牙囊细胞CSF—1受体mRNA表达无影响。

Colony-stimulating factor-1 receptor in rat dental follicle cells

OBJECTIVE: To study localization and expression of CSF-1 receptor protein, in order to discover the CSF-1 and IL-1alpha effects on CSF-1 receptor mRNA levels and to determine if the autocrine effect is inhibited through the CSF-1 receptor. METHODS: Immunolocalization of CSF-1 receptor in the cultured dental follicle cells and in mandibles of the post-natal rats from day 1 to 11 were performed. The effects of different concentrations of CSF-1, IL-1alpha on CSF-1 receptor gene expression were detected by means of RT-PCR. RESULTS: Cultured dental follicle cells were immunostained for the CSF-1 receptor. In vivo, immunostaining showed that the CSF-1 receptor was present in the dental follicle of the first mandibular molar at early post-natally and was either absent or greatly reduced by day 11 post-natally. High concentrations of cvCSF-1 reduced the gene expression of the CSF-1 receptor. IL-1alpha had no effects on CSF-1 receptor mRNA levels. CONCLUSIONS: The expression of CSF-1 receptor reaches a peak early post-natally in the dental follicle of the first mandibular molar of the rat and then subsequently declines. High concentrations of CSF-1 inhibits the expression of CSF-1 receptor, IL-1alpha has no effect on the expression of CSF-1 receptor mRNA.