Similarity between Spatial-temporal Expression Patterning of HOXAll and Progesterone Receptor in Human Endometrium

11llPlantation 15 a eomPlex series of Proeesses,whieh establish the connection betweenmaternal and exz〕bryonie tissue.The suceessful imPlantation of the human ell〕I〕ryo dePendson the synehronous develoPment of a reeePtive endometrium.The Proliferation and differ-entiation of the endometrium during menstrual eyele are neeessary for establishing reeeP-tive state.Although a lot of information 15 eurrently available,the moleeular meehanismsthat direet the ordered Proliferation and different…

Similarity between Spatial-temporal Expression Patterning of HOXAll and Progesterone Receptor in Human Endometrium

Objective To study HOXAll expression and its correlation with that of the progesterone receptor (PR) gene in human endometriumMethods In situ hybridization and semi-quantitative RT-PCR were used. Results HOXAll mRNA was detected in both stromal and glandular cells of normal endometrium by in situ hybridization. But the expression levels in the glandular cells had a dramatic decline or even disappearance at mid-secretory stage. Semi-quantitative RT-PCR analysis, however, demonstrated that the total expression levels of HOXAll mRNA were markedly increased in the mid-secretory endometrium, which suggested that there was an increased expression in stromal cells. Similar results were obtained for PR gene expression in human endometrium by in situ hybridization and semi-quantitative RT-PCR analysis.Conclusion HOXAll gene spatial and temporal expression patterns were similar to that of PR gene in endometrium across menstrual cycle, and HOXAll was closely related to the endometrial proliferation and differentiation during menstrual cycle, especially the establishment of receptive status in implantation.