| PCSK9-siRNA对ox-LDL诱导的THP-1源性巨噬细胞凋亡中Caspase-3表达的影响 |
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| 引用本文： | 唐志晗,武春艳,任重,刘录山,姜志胜.PCSK9-siRNA对ox-LDL诱导的THP-1源性巨噬细胞凋亡中Caspase-3表达的影响[J].广西医科大学学报,2011,28(1):58-61. |
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| 作者姓名： | 唐志晗 武春艳 任重 刘录山 姜志胜 |
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| 作者单位： | 南华大学心血管病研究所动脉硬化湖南省重点实验室,衡阳,421001 |
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| 基金项目： | 湖南省应用基础研究计划重点项目，湖南省科技厅计划项目，湖南省教育厅重点科研项目，湖南省高校科技创新团队支持计划资助 |
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| 摘 要： | 目的:研究枯草溶菌素转化酶9 (PCSK9)-siRNA对ox-LDL诱导的THP-1源性巨噬细胞凋亡中Caspase-3蛋白表达的影响.方法:用不同浓度.x-LDL处理THP-1源性巨噬细胞不同时间,Western blot检测Caspase-3蛋白表达变化.应用Li-pofectamine2000分别转染不同浓度P...
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| 关 键 词： | 枯草溶菌素转换酶9 巨噬细胞 凋亡 Caspase-3 |
EFFECT OF PCSK9-siRNA ON THE EXPRESSION OF Caspase-3 IN ox-LDL-INDUCED THP-1 MACROPHAGE CELLS |
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| Tang Zhihan,Wu Chunyan,Ren Zhong,Liu Lushan,Jiang Zhisheng.EFFECT OF PCSK9-siRNA ON THE EXPRESSION OF Caspase-3 IN ox-LDL-INDUCED THP-1 MACROPHAGE CELLS[J].Journal of Guangxi Medical University,2011,28(1):58-61. |
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| Authors: | Tang Zhihan Wu Chunyan Ren Zhong Liu Lushan Jiang Zhisheng |
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| Institution: | .(Institute of Cardiovascular Disease,Key Lab for Arteriosclerology of Hunan Province,University of South China,Hengyang 421001,China) |
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| Abstract: | Objective：To demonstate the ffect of PCSK9-siRNA on the expression of Caspase-3 in ox-LDL-induced THP-1 macrophage cells.Methods：Different concentrations of ox-LDL were used to treat THP-1 macrophages at different times.Western blot detected Caspase-3 protein level.Lipofectamine2000 were transfected with different concentrations PCSK9 siRNAs（30,50,80 nmol/L） into THP-1 derived macrophages.After 24 hours,ox-LDL was added for 48 hours,Western blot examined Caspase-3 expression.Results：Ox-LDL increased Caspase-3 protein expression in a dose and time dependent manner.PCSK9-siRNA significantly reduced Caspase-3 protein expression in a dose dependent manner.Conclusion：PCSK9-siRNA inhibited the apoptosis of ox-LDL-induced THP-1 derived macrophages.The mechanism of this action may be related to the downregulation of Caspase-3. |
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| Keywords: | Caspase-3 |
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