| Attenuation of Telomerase Activity by siRNA Targeted Telomerase RNA Leads to Apoptosis and Inhibition of Proliferation in Human Renal Carcinoma Cells |
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| 作者姓名： | Rumin Wen Junjie Liu Wang Li Wenfa Yang Lijun Mao Junnian Zheng |
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| 作者单位： | Rumin Wen Junjie Liu Wang Li Wenfa Yang Lijun Mao Junnian Zheng Laboratory of Urology，Affiliated Hospital of Xuzhou Medical College，Xuzhou 221002，China. |
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| 摘 要： | OBJECTIVE Telomerase is an attractive molecular target for cancer therapy because the activation of telomerase is one of the key steps in cell immortalization and carcinogenesis. RNA interference using small-interfering RNA (siRNA) has been demonstrated to be an effective method for inhibiting the expression of a given gene in human cells. The aim of the present study was to investigate whether inhibition of telomerase activity by siRNA targeted against human telomerase RNA (hTR) can inhibit proliferation and induce apoptotic cell death in human renal carcinoma cells (HRCCs). METHODS The siRNA duplexes for hTR were synthesized and 786-0 HRCCs were transfected with different concentrations of hTR-siRNA. The influence on the hTR mRNA level, telomerase activity, as well as the effect on cell proliferation and apoptosis was examined. RESULTS Anti-hTR siRNA treatment of HRCCs resulted in specific reduction of hTR mRNA and inhibition of telomerase activity. Additionally, significant inhibition of proliferation and induction of apoptosis were observed. CONCLUSION siRNA against the hTR gene can inhibit proliferation and induce apoptosis by blocking telomerase activity of HRCCs. Specific hTR inhibition by siRNA represents a promising new option for renal cancer treatment.
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| 关 键 词： | 端粒酶 酶活性 细胞凋亡 肾肿瘤 |
| 收稿时间： | 2006-07-05 |
| 修稿时间： | 2006-10-10 |
Attenuation of telomerase activity by siRNA targeted telomerase RNA leads to apoptosis and inhibition of proliferation in human renal carcinoma cells |
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| Rumin Wen Junjie Liu Wang Li Wenfa Yang Lijun Mao Junnian Zheng.Attenuation of Telomerase Activity by siRNA Targeted Telomerase RNA Leads to Apoptosis and Inhibition of Proliferation in Human Renal Carcinoma Cells[J].Chinese Journal of Clinical Oncology,2006,3(5):326-331. |
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| Authors: | Rumin Wen Junjie Liu Wang Li Wenfa Yang Lijun Mao Junnian Zheng |
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| Institution: | Laboratory of Urology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China |
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| Abstract: | OBJECTIVE Telomerase is an attractive molecular target for cancer therapy because the activation of telomerase is one of the key steps in cell immortalization and carcinogenesis. RNA interference using small-interfering RNA (siRNA) has been demonstrated to be an effective method for inhibiting the expression of a given gene in human cells. The aim of the present study was to investigate whether inhibition of telomerase activity by siRNA targeted against human telomerase RNA (hTR) can inhibit proliferation and induce apoptotic cell death in human renal carcinoma cells (HRCCs). METHODS The siRNA duplexes for hTR were synthesized and 786-0 HRCCs were transfected with different concentrations of hTR-siRNA. The influence on the hTR mRNA level, telomerase activity, as well as the effect on cell proliferation and apoptosis was examined. RESULTS Anti-hTR siRNA treatment of HRCCs resulted in specific reduction of hTR mRNA and inhibition of telomerase activity. Additionally, significant inhibition of proliferation and induction of apoptosis were observed. CONCLUSION siRNA against the hTR gene can inhibit proliferation and induce apoptosis by blocking telomerase activity of HRCCs. Specific hTR inhibition by siRNA represents a promising new option for renal cancer treatment. |
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| Keywords: | human telomerase RNA telomerase small-interfering RNA renal cell carcinoma proliferation |
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