| LC-MS鉴定肝毒性吡咯里西啶类生物碱千里光碱大鼠体内代谢产物 |
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| 引用本文： | 熊爱珍,杨莉,张芳,何芋岐,王长虹,王峥涛. LC-MS鉴定肝毒性吡咯里西啶类生物碱千里光碱大鼠体内代谢产物[J]. 中国药学杂志, 2012, 47(1): 54-60 |
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| 作者姓名： | 熊爱珍 杨莉 张芳 何芋岐 王长虹 王峥涛 |
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| 作者单位： | 1.上海中医药大学中药研究所，a.中药标准化教育部重点实验室；b.上海市复方中药重点实验室，上海 201203；2.中国药科大学生药学研究室，南京 210038 |
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| 基金项目： | “重大新药创制”科技重大专项(2009ZX09308-003,2009ZX09052-021,2009ZX09052-024);国家自然科学基金(30701104);上海市国际合作基金项目(09540704700) |
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| 摘 要： | 目的 研究肝毒性吡咯里西啶类生物碱千里光碱的大鼠体内代谢。方法 SD大鼠口服千里光碱，收集胆汁、尿液及粪便样本，采用液相色谱-多级质谱联用对代谢产物进行研究，推导其体内代谢模式。结果 正负离子下共检测到38种代谢产物，主要为千里光碱及其代谢物的氮氧化物，羟化产物，水解产物，以及硫酸结合物，葡萄糖醛酸结合物和谷胱甘肽结合物。另外，尿液、胆汁与粪便中检测到的代谢物有一定差异，胆汁和尿液中代谢产物较多。结论 千里光碱在大鼠体内代谢广泛，形成多种代谢产物。
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| 关 键 词： | 吡咯里西啶类生物碱 千里光碱 多级质谱 体内代谢 |
Identification of Metabolites of Senecionine,a Hepatotoxic Pyrrolizidine Alkaloid,by Liquid Chromatography/Tandem Mass Spectrometry |
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| XIONG Ai-zhen,a,YANG Li,a,ZHANG Fang,a,HE Yu-qi,a,WANG Chang-hong,a,WANG Zheng-tao. Identification of Metabolites of Senecionine,a Hepatotoxic Pyrrolizidine Alkaloid,by Liquid Chromatography/Tandem Mass Spectrometry[J]. Chinese Pharmaceutical Journal, 2012, 47(1): 54-60 |
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| Authors: | XIONG Ai-zhen a YANG Li a ZHANG Fang a HE Yu-qi a WANG Chang-hong a WANG Zheng-tao |
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| Affiliation: | 1a,1b,2(1a.The MOE Key Laboratory for Standardization of Chinese Medicines,1b.The Shanghai Key Laboratory for Compound Chinese Medicines,Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;2.Department of Pharmacognosy,China Pharmaceutical University,Nanjing 210038,China) |
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| Abstract: | OBJECTIVE To investigate the metabolic profile of senecionine in rats.METHODS Liquid chromatography-tandem mass spectrometry(LC-MSn) mass spectrometry was used for the analysis of senecionine and its metabolites in rats bile,urine,and feces after a single oral dose of senecionine.RESULTS In total 38 metabolites were identified,including oxidation products,hydroxylation products,hydrolysis products,sulfation products,glucuronidation products,and GSH-conjugations.And metabolites were found to be more abundant in bile and urine than those in feces.CONCLUSION Senecionine undergoes extensive metabolism in rats. |
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| Keywords: | hepatotoxic pyrrolizidine alkaloid senecionine tandem mass in vivo metabolism |
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