| NP-HPLC法拆分特拉唑嗪对映体 |
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| 引用本文： | 刘曼,张丹,韩静,刘会臣.NP-HPLC法拆分特拉唑嗪对映体[J].中国医药工业杂志,2012,43(1):53-56. |
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| 作者姓名： | 刘曼 张丹 韩静 刘会臣 |
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| 作者单位： | 航天中心医院临床药理室,北京,100049 |
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| 摘 要： | 建立了正相高效液相色谱法拆分特拉唑嗪对映体.采用Chiralpak AD-H手性色谱柱,考察了流动相组成、流速及柱温对对映体分离的影响.确定最佳拆分条件:流动相为正己烷-异丙醇-二乙胺(65∶35∶0.1),流速0.7 ml/min,检测波长254 nm,柱温25℃.在此条件下特拉唑嗪对映体的分离度为3.1.两对映体在2.5～7.5 μg/ml浓度范围内线性关系良好,平均回收率为99.8％和99.4％,RSD为0.44％和0.42％.
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| 关 键 词： | 特拉唑嗪 手性固定相 正相高效液相色谱 手性拆分 |
Enantiomeric Separation of Terazosin by NP-HPLC |
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| Authors: | LIU Man ZHANG Dan HAN Jing LIU Huichen |
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| Institution: | *(Dept.of Clinical Pharmacology,Aerospace Center Hospital,Beijing 100049) |
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| Abstract: | A NP-HPLC method was established for the enantiomeric separation of terazosin.Baseline chiral separation of terazosin enantiomers was achieved under normal-phase mode using the Chiralpak AD-H chiral column.The influences of the composition of the mobile phase,the flow rate and column temperature on the enantiomeric separation were investigated.The optimal chromatographic conditions were hexane-isopropyl alcohol-diethylamine(65︰35︰0.1) as the mobile phase at the flow rate of 0.7 ml/min and the detection wavelength of 254 nm at the column temperature of 25 ℃.The resolution between the peaks of terazosin enantiomers was 3.1 under the above chromatographic conditions.The calibration curves of terazosin enantiomers were linear in the concentration range of 2.5-7.5 μg/ml.The average recoveries were 99.8% and 99.4%,with RSDs of 0.44% and 0.42%. |
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| Keywords: | terazosin chiral stationary phase NP-HPLC chiral separation |
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