阿托伐他汀对内皮细胞微粒诱导人脐静脉内皮细胞VCAM-1和ICAM-1表达的影响

目的:探讨阿托伐他汀对内皮细胞微粒(EMPs)诱导的人脐静脉内皮细胞(HUVECs)表达血管细胞粘附分子(VCAM)-1和细胞间粘附分子(ICAM)-1的影响。方法:取生长良好的第4,5代人脐静脉内皮细胞,将细胞分为3大组:对照组、EMPs组、EMPs+阿托伐他汀组。对照组加入培养基,EMPs组以不同浓度的EMPs(0/mL,1×102/mL,1×103/mL,1×104/mL,1×105/mL)与HUVECs共同孵育24 h,EMPs+阿托伐他汀组以不同浓度的阿托伐他汀(0.05,0.1,1.0,10μmol.L-1)与HUVECs作用1 h后,加入105/mL EMPs共同孵育24 h。分别采用实时荧光定量聚合酶链反应和蛋白免疫印迹方法检测VCAM-1和ICAM-1 mRNA和蛋白的表达。结果:HUVECs受EMPs刺激后,VCAM-1和ICAM-1 mRNA及蛋白表达呈浓度依赖性增加,阿托伐他汀可不同程度上抑制EMPs的作用。结论:阿托伐他汀抗动脉粥样硬化作用可能部分与抑制EMPs诱导的内皮细胞VCAM-1和ICAM-1的表达有关。

Effects of atorvastatin on the expression of VCAM-1 and ICAM-1 in human umbilical vein endothelial cells induced by endothelial microparticles

OBJECTIVE To explore the effects of atorvastatin on the expression of vascular cell adhesion molecule-1(VCAM-1) and intercellular adhesion molecule 1(ICAM-1) in human umbilical vein endothelial cells induced by endothelial microparticles.METHODS Human umbilical vein endothelial cells(HUVECs) at passage 4 to 5 were divided into three groups: control,EMPs,atorvastatin group.HUVECs in EMPs group were incubated with different concentrations of EMPs(0/mL,1×102/mL,1×103/mL,1×104/mL,1×105/mL) for 24 h.HUVECs in atorvastatin group were cultured with different concentrations of atorvastatin(0.05,0.1,1.0,10 μmol·L-1)for 1h before incubated with EMPs(1×105/mL) for 24 h.Quantitative real-time polymerase chain reaction and Western blotting were used to detect expression of mRNA and protein of ICAM-1 and VCAM-1 respectively.RESULTS Both two detection methods showed that endothelial microparticles stimulation significantly induced the mRNA and protein expression of VCAM-1 and ICAM-1 in HUVECs in a dose-dependent manner.The increase of VCAM-1 and ICAM-1 mRNA and protein expression induced by EMPs was inhibited by atorvastatin.CONCLUSION The anti-atherosclerotic effects of atorvastatin partly attributed to inhibition of VCAM-1 and ICAM-1 induced by EMPs.