| 3种旋毛虫成虫永久标本染色方法的比较 |
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| 引用本文： | 杨丁,皮本伟,李丹,牛利娜,张莹,王国英,赵粤萍.3种旋毛虫成虫永久标本染色方法的比较[J].中国病原生物学杂志,2012(1):46-47. |
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| 作者姓名： | 杨丁 皮本伟 李丹 牛利娜 张莹 王国英 赵粤萍 |
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| 作者单位： | 河南大学医学院;河南大学医学院免疫学研究所 |
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| 基金项目： | 河南大学大学生创新性实验资助课题(No.2010-10NB032) |
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| 摘 要： | 目的探讨制作旋毛虫成虫染色标本的最佳方法。方法分别使用乙醇硼砂卡红、醋酸卡红、固绿3种染色法制作旋毛虫成虫标本。结果乙醇硼砂卡红染色的标本,颜色鲜艳,结构清晰,另外两种染液染色效果不佳。结论乙醇硼砂卡红染色可用于旋毛虫成虫永久标本的制作。
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| 关 键 词： | 旋毛虫 成虫 标本制作 |
A comparative study of three methods of staining to prepare permanent specimens of adult Trichinella spiralis worms |
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| Institution: | YANG Ding,PI Ben-wei,LI Dan,et al(Medical College,Henan University,Kaifeng 475004,Henan,China) |
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| Abstract: | Objective To determine the best method of staining to prepare permanent specimens of adult Trichinella spiralis worms.Methods Alcohol-borax-carmine,acetic acid carmine,and fast green were each used to prepare specimens of adult T.spiralis worms.Results Specimens stained with alcohol-borax-carmine were brighter and had a more readily apparent internal structure than specimens stained by other methods.Conclusion Alcohol-borax-carmine staining can be used to prepare permanent specimens of adult T.spiralis worms. |
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| Keywords: | Trichinella spiralis adult worms specimen preparation |
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