抗环瓜氨酸肽抗体的定量检测在类风湿性关节炎诊断中的应用

目的 探讨抗环瓜氨酸肽(CCP)抗体在类风湿性关节炎(RA)中的诊断意义。方法 采用酶联免疫吸附法(ELISA)定量检测抗CCP抗体相对浓度，采用免疫散射比浊法定量检测类风湿因子(RF)浓度，并比较抗CCP抗体与RF的相关性。结果 抗CCP抗体在RA诊断中其敏感性较RF低(P＜0．05)，特异性较RF高，有显著性差异(P＜0．01)，抗CCP抗体联合检测在诊断RA时，能使敏感性提高到86．7％，特异性提高到98．6％。结论 抗CCP抗体对RA具有很高的特异性。尚不能认为与RF具有相关性。因此，两者联合检测能提高RA血清学的阳性率。

Diagnostic value of anti-cyclic citrullinated peptide antibody quantitative analysis in patients with rheumatoid arthritis

Objective To explore the significance of anti-cyclic citrullinated peptide(CCP) antibody in the diagnosis of rheumatoid arthritis. Methods The specificity and sensitivity of anti-CCP were detected by ELISA. The specificity and sensitivity of rheumatoid factor were detected by nephelometry. The relativity between anti-CCP and RF was compared. Results The results were showed significant difference by using anti-cyclic citrullinated petide antibody(P<0.01), and the c-detection of anti-cyclic citrullinated peptide antibody and rheumatoid factor can increase sensility and speciality in diagnosing rheumatoid arthritis. Conclusions The CCP antibody has very high specificity. It is not obvious correlation between anti-CCP and rheumatoid factor(r=0.2548, P>0.05). Determination of anti-CCP antibody in addition to RF can increase the positive diagnostic rate in RA.