K+通道在Jurkat细胞调节性体积减小中的作用

目的：研究K^ 通道在Jurkat细胞调节性体积减小(RVD)中的作用。方法：调节细胞外不同离子浓度，用细胞成像系统测定Jurkat细胞(人T淋巴细胞肿瘤细胞株)在不同渗透压时细胞容积的变化。结果：细胞培养液中缺少Ca2^ ，或细胞培养液中的K^ 浓度升高，或使用Ca^2 敏感的K^ 通道(KCa)阻断剂均可抑制Jurkat细胞的RVD；应激免疫抑制蛋白(ISPS)具有抑制Jurkat细胞RVD的作用。结论：Jurkat细胞中KCa通道是RVD不可缺少的因素。ISPS对免疫功能抑制作用的机理之一，可能是抑制了T淋巴细胞上的K^ 通道。

Effects of potassium ion channel on regulatory volume decrease in Jurkat cells

AIM: To study the effect of K + channel on the regulatory volume decrease (RVD) in Jurkat cells. METHODS: An imaging analysis system was used for the measurement of cell volume under the conditions of hypotonic stress in Jurkat cells. RESULTS: RVD in Jurkat cells was found to be inhibited under the conditions of lack of Ca 2+ in the medium, and increased in concentration of K + or administration of KCa channel blocker. Immune suppressive protein of stress (ISPS) was also able to inhibit the RVD in Jurkat cells. CONCLUSION: KCa channel plays an important role in RVD in Jurkat cells. The suppression on K + channel may be one of the possible reasons for the inhibition on immune function induced by ISPS.