Glucocorticoids increase the 1,25(OH)2D3 receptor concentration in rat osteogenic sarcoma cells |
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Authors: | Stavros C Manolagas Jean Abare Leonard J Deftos |
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Institution: | (1) Department of Medicine, University of California, 92037 San Diego, La Jolla, California |
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Abstract: | Summary We have used cultured osteoblastlike rat osteogenic sarcoma cells (ROS 17/2) which have receptors for 1,25(OH)2D3 and for glucocorticoids, and have examined the modulation of the 1,25(OH)2D3 receptor by the potent glucocorticoid triamcinolone acetonide. We report that triamcinolone acetonide caused an increase
of the 1,25(OH)2D3 receptor concentration in these cells but it did not affect the affinity of the receptor to 1,25(OH)2D3; this phenomenon occurred in a dosedependent fashion for triamcinolone (10−9 to 10−7 M) with a maximum increase of 1,25(OH)2D3 receptor concentration of ⋍twofold. During the culture period, the 1,25(OH)2D3 receptor concentration was altered both in untreated as well as in triamcinolone-treated cells, being highest at the early
logarithmic phase and diminished progressively as cells approached confluence. However, throughout the culture period, the
1,25(OH)2D3 receptor concentration was higher in the triamcinolone-treated cells. |
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Keywords: | 1 25(OH)2D3 Glucocorticoids Triamcinolone acetonide Receptors |
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