胰腺癌细胞对5-Fu和吉西它滨获得性耐药后bcl-xL和mcl-1表达的变化

目的 :探讨胰腺癌细胞对 5 Fu和吉西它滨的获得性耐药后bcl xL 和mcl 1表达的变化。方法 :应用SRB方法检测 5 Fu和吉西它滨对胰腺癌细胞株Panc 1,Mia Paca 2和Capan 1的细胞毒性作用 ,应用Westernblot法来检测bcl xL 和mcl 1的蛋白表达水平。结果 :5 Fu和吉西它滨对 3株胰腺癌细胞均产生了细胞毒性作用 ,5 Fu长期作用后 ,Capan 1细胞IC50 上升了 2 .1倍 (P <0 .0 5 )。吉西它滨长期作用后 ,Capan 1细胞IC50 上升了 1.5倍 (P <0 .0 5 )。 5 Fu或吉西它滨长期作用后 ,产生了获得性耐药的胰腺癌细胞bcl xL 和mcl 1表达水平上调。结论 :5 Fu或吉西它滨长期作用后 ,产生了获得性耐药 ,这种耐药与bcl xL 和mcl 1的过度表达相关。

The expression of bcl-xL and mcl-1 following acquired chemoresistance in pancreatic cancer cells

Objective:To study the relationships between the acquired resistance of Gemcitabine and 5 Fu and the expression of bcl x L and mcl 1 in pancreatic cancer cell lines.Methods:The cytotoxic effects of 5 Fu and Gemcitabine in Panc 1,Mia Paca 2 and Capan 1 pancreatic cancer cells were assessed using Sulforhodamine B methods and expression levels of anti apoptotic genes of the bcl x L and mcl 1 were analyzed by Western blot.Results:5 Fu and Gemcitabine displayed cytotoxic effects towards three pancreatic cancer cell lines.After recurrent treatment with 5 Fu,the IC 50 values in Capan 1 cells increased 2.1 fold compared to their parental cells (P<0.05).Following recurrent treatment with Gemcitabine,the IC 50 values in Capan 1 cells increased 1.5 fold (P<0.05).Western blot analysis show that aftter recurrent treatment with 5 Fu and Gemcitabine the expression of bcl x L and mcl 1 upgraded.Conclusion:Overexpression of bcl x L and mcl 1 following recurrent exposure to 5 Fu or Gemcitabine are associaed with the acqurired these drugs