复方苦参注射液对泪腺腺样囊性癌ACC-2细胞增殖、凋亡及Caspase-3蛋白表达的影响

目的探讨复方苦参注射液对泪腺腺样囊性癌ACC-2细胞增殖、凋亡及Caspases-3蛋白表达的影响。方法体外培养人泪腺腺样囊性癌ACC-2细胞,应用MTT法检测细胞增殖;AnnexinV/PI双染色流式细胞仪检测细胞凋亡和细胞周期;ELISA法检测Caspases-3蛋白表达。结果复方苦参注射液对ACC-2细胞的体外增殖具有抑制作用,量效关系显著,与对照组比较有统计学差异(P<0.01),半数抑制浓度(IC50)为0.84g/ml。经流式细胞仪检测表明,复方苦参注射液能使ACC-2细胞G0-G1期逐渐增加,G2-M期和S期逐渐减少,并且随着剂量的增加,ACC-2细胞凋亡率明显增加(P<0.05或P<0.01)。复方苦参注射液能增强ACC-2细胞Caspases-3蛋白的表达(P<0.05或P<0.01),并呈剂量依赖性。结论复方苦参注射液能有效抑制人泪腺腺样囊性癌ACC-2细胞Caspases-3蛋白表达,诱导ACC-2细胞凋亡,抑制肿瘤细胞增殖。

Effects of Compound Radix Sophorae Flavescentis injection on proliferation, apoptosis and Caspase-3 expression in adenoid cystic carcinoma ACC-2 cells

Objective To investigate the effects of compound Radix Sophorae Flavescentis injection on proliferation, apoptosis and Caspase-3 expression in human adenoid cystic carcinoma ACC-2 cells. Methods ACC-2 cells were cultured in vitro. MT＇I＂ assay was used to measure the cell proliferative effect. The Annexin V/PI double staining analysis by flow cytometry was used to evaluate apoptotic rate and the cell cycle. The expression of Caspases-3 protein was detected by enzyme-linked immunosorbent assay （ELISA）. Results Compound Radix Sophorae Flavescentis injection could inhibit the proliferation of ACCo2 cells in vitro, and dose-effect relationship was significant（ P〈0.01 ）. IC50 of ACC-2 was 0.84 g/ml. The flow cytometry test indicated, compound Radix Sophorae Flavescentis injection could make ACC-2 cells G0-G1 phase gradually increasing, G2-M period and S phase reduce gradually, and with the increase of the dose, ACC-2 cell apoptosis rate increased significantly （P〈0.05 or P〈0.01 ）. Compound Radix Sophorae Flavescentis injection could enhance ACC-2 cells Caspases-3 protein expression （P〈0.05 or P〈0.01 ）, and with the dose dependent. Conclusion Compound Radix Sophorae Flavescentis injection could effectively restrain human adenoid cystic carcinoma ACC-2 cells Caspases-3 protein expression, induction ACC-2 cells apoptosis, inhibiting tumor cell proliferation.