MR示踪技术在干细胞治疗心肌梗死中的研究进展

活体细胞MR示踪成像技术能有效在体内实时、准确评价干细胞治疗心肌梗死的效果,可分为MR对比剂标记细胞成像和MR报告基因成像。自体移植干细胞治疗心肌梗死的疗法中,能标记干细胞的MR对比剂主要有以钆剂为主的顺磁性对比剂、化学交换饱和转移成像(CEST)相关对比剂以及氧化铁类对比剂;MR报告基因导入后能使细胞表达产生MR信号改变的蛋白质,以膜表面蛋白为主,包括铁蛋白受体、膜表面抗原、酶等。目前的MR细胞示踪技术对自体移植干细胞治疗的疗效评价、机制研究有一定的指导作用。

Progress of MRI techniques in tracking of stem cells for myocardial infarction

MRI-associated techniques for in-vivo tracking of stem cells can be used to effectively and accurately evaluate stem cell therapy for myocardial infarction in real-time. It includes MRI contrast agent (CA) labeling technique and MR reporter gene editing tool. MR contrast agents which are capable of labeling stem cells in treatment of myocardial infarction mainly include positive contrast agents (mainly gadolinium-based paramagnetic contrast agents), chemical exchange saturation transfer(CEST)-MRI imaging related contrast agents, and iron oxide-based agents. After the introduction of the MR reporter gene, the cells can express proteins that produce MR signal changes, mainly membrane proteins, including ferritin receptors, membrane surface antigens, and enzymes. At present, the existing MR cell tracking techniques has a certain guiding role in the evaluation and mechanism study of autologous transplantation stem cell therapy.