表皮生长因子协同体对大脑中动脉闭塞性脑梗死急性期的保护性作用研究

目的研究表皮生长因子协同体(EGFC)对大鼠大脑中动脉闭塞(MCAO)急性期的作用,为其临床转化提供理论基础和实验依据。方法选取SD大鼠随机分为Sham组、MCAO组、低剂量EGFC组(2μg)、中剂量EGFC组(20μg)、高剂量EGFC组(200μg)。制备MCAO模型,缺血2 h后再灌注,3 h后腹腔给药,24 h后处死,采用TTC染色测定梗死体积;测定脑组织含水量,计算湿干比; ELISA检测脑组织中TNF-α、IL-1β、IL-6等炎症因子的表达水平。结果与MCAO组相比,不同剂量EGFC组梗死体积均减小,当给予EGFC 200μg时,差异有统计学意义(P 0. 01)。与MCAO组相比,不同剂量EGFC组脑组织含水量均降低,且差异有统计学意义(P 0. 05)。ELISA检测炎性因子,与Sham组相比,MCAO组和低剂量EGFC组中TNF-α、IL-1β、IL-6的含量明显升高,差异具有统计学意义(P 0. 01);与MCAO组相比,中、高剂量EGFC组3种炎症因子的含量均降低,差异均有统计学意义(P 0. 05);与低剂量EGFC组相比,中、高剂量EGFC组3种炎症因子的含量均降低,差异均有统计学意义(P 0. 05,P 0. 01)。结论 EGFC对MCAO大鼠脑组织具有保护作用,且在一定范围内,随着给药剂量的增加,保护作用也增强。

Protective effect of epidermal growth factor coordination in the acute stage of middle cerebral artery occlusion

Objective To investigate the effect of epidermal growth factor coordination (EGFC) in the acute stage of middle cerebral artery occlusion (MCAO) in rats, and to provide a theoretical and experimental basis for bench to bedside translation. Methods Sprague-Dawley rats were randomly divided into sham group, MCAO group, low-dose (2 μg) EGFC group, medium-dose (20 μg) EGFC group, and high-dose (200 μg) EGFC group. A rat model of MCAO was established by 2 hours of ischemia followed by reperfusion. Rats received intraperitoneal administration of EGFC at 3 hours after model establishment and were sacrificed at 24 hours. TTC staining was used to measure infarct volume. The brain water content and wet/dry ratio were determined. Enzyme-linked immunosorbent assay was used to evaluate the expression levels of inflammatory factors, i.e., tumor necrosis factor α (TNF-α), interleukin 1β (IL-1β), and interleukin 6 (IL-6). Results Compared with the MCAO group, all EGFC groups had reduced infarct volume, in which the high-dose EGFC group had significantly reduced infarct volume (P<0.01). All EGFC groups had significantly lower brain water content than the MCAO group (P<0.05). According to the ELISA results, the MCAO group and the low-dose EGFC group had significantly higher levels of TNF-α, IL-1β, and IL-6 than the sham group (P<0.01); the medium-and high-dose EGFC groups had significantly lower levels of TNF-α, IL-1β, and IL-6 than the MCAO group (P<0.05) and the low-dose EGFC group (P<0.05, P<0.01). Conclusions EGFC has a protective effect on the brain of MCAO rats. Within a certain range, the EGFC dose is positively correlated with the protective effect.