α-常春藤皂苷一氧化氮供体衍生物的合成及其抗肿瘤活性研究

目的合成并表征α-常春藤皂苷一氧化氮供体衍生物,并对其抗肿瘤活性进行研究。方法采用苯硫酚作为起始原料,以不同长度的二醇作为连接臂,将α-常春藤皂苷的28位羧基和呋咱氮氧化物连接在一起得到α-常春藤皂苷一氧化氮供体衍生物;并采用MTT法对目标化合物进行体外抗乳腺癌活性研究。结果合成了4个α-常春藤皂苷一氧化氮供体衍生物,其结构均通过1H-NMR和MS确证。生物活性结果测试表明其抗肿瘤活性均优于阳性对照药α-常春藤皂苷。结论α-常春藤皂苷一氧化氮供体衍生物抗肿瘤活性明显,具有很好的开发价值。

Discussion on GLP process and key points in the histopathology peer review for nonclinical toxicology studies

Histopathology assessment is an important step for the determination and interpretation of the results of nonclinical toxicology studies. Histopathology peer review can ensure the consistency, accuracy of pathological diagnosis terms and the completeness of pathological diagnosis, and can improve accuracy of histopathology assessment and the quality of pathology reports. Because the histopathology peer review processes need to be considered the requirements of the GLP regulations of different countries, therefore, it is necessary to deeply understand and summarize the GLP compliance, processes, conditions and considerations of histopathology peer review so as to promote the consistency in planning, conducting, documenting and reporting of the histopathology peer review in China with developed countries abroad as soon as possible. This article introduced the purpose and necessity of carrying out the histopathology peer review, the GLP principles of histopathology peer review, the GLP quality system applicable to the histopathology peer review, the processes and conditions of histopathology peer review, as well as the key points for the histopathology peer review, with the purpose of providing some references for better carrying out histopathology peer review in nonclinical safety evaluation facilities for drugs in China.