肾综合征出血热尸检组织中HFRSV抗原分布的研究

应用双桥 PAP 法和 ABC 法对12例肾综合征出血热尸检组织(1962～1986年)的 HFRSV抗原检测发现病毒抗原广泛分布于各组织中,所检测过的大脑、肾、心、肺、肝、脾、胰、肾上腺等主要脏器大部分病例为明显阳性,少数病例还保存有胃、支气管、小脑、脑垂体、甲状腺、淋巴结和扁桃体,经检测部分病例也为阳性。各组织中的阳性细胞主要为小血管和毛细血管内皮细胞,肾小管上皮细胞、胰腺、脑垂体、肾上腺和甲状腺上皮细胞部分病例明显阳性,另外,各组织中病毒抗原量与病程发展(病期)有一定的关系。

The Distribution of HFRSV Antigen in Old Paraffin Sections from Autopsy Specimens by Immunohistochemical Methods

The tissues from 12 cases of hemorrhagic fever with renal syndrome (HFRS)were obstained be- tween 1962 and 1986 from autopsy specimens.The distribution of HFRS viral (HFRSV) antigen in the tissues was determined utilizing a double bridge peroxidase antiperoxidase(PAP)method combined with an avidin biotin conjugate (ABC)peroxidase technique.HFRSV antigen was usually found in brain,kidney,heart,lung,liver,spleen,pituitary,adrenal gland and pancreas.HFRSV antigen was also examined in stomach,trachea,cerebellum,thyroid,lymph nodes and tonsils in some of the cases.The positive cells were found mainly in endothelium of capillaries and small blood vessels,and in macrophages everywhere.There were fewer positive parenchymal cells.However,epithelia of renal tubules,alveolar cells of pancereas,and some endocrine glands of a part of cases were positive.In addi- tion,it was found that there was the relationship between the extent of viral antigen in the tissues and the disease phase.