| 磺胺胍和磺胺噻唑人工抗原的合成与鉴定 |
| |
| 引用本文： | 崔艳华,张海玲,马莺,代翠红,崔杰.磺胺胍和磺胺噻唑人工抗原的合成与鉴定[J].卫生研究,2010,39(4). |
| |
| 作者姓名： | 崔艳华 张海玲 马莺 代翠红 崔杰 |
| |
| 作者单位： | 哈尔滨工业大学食品科学与工程学院食品科学系,哈尔滨,150090 |
| |
| 基金项目： | 哈尔滨市科技攻关计划项目 |
| |
| 摘 要： | 目的制备磺胺胍和磺胺噻唑的人工抗原以检测动物性食品中的磺胺胍和磺胺噻唑。方法通过重氮化法将磺胺胍和磺胺噻唑与牛血清白蛋白(BSA)偶联,制备磺胺胍和磺胺噻唑人工抗原,偶联比分别为10∶1和9∶1。用该抗原免疫小鼠,间接ELISA法检测抗血清效价。结果经过紫外光谱检测、红外光谱检测及免疫动物实验确证该人工抗原。结论磺胺胍和磺胺噻唑人工抗原合成成功,为获得抗磺胺胍和磺胺噻唑的特异性抗体奠定了基础。
|
| 关 键 词： | 磺胺胍 磺胺噻唑 人工抗原 |
Synthesis and identification of artificial antigens for sulfaguanidine and sulfathiazole |
| |
| CUI Yanhua,ZHANG Hailing,MA Ying,DAI Cuihong,CUI Jie.Synthesis and identification of artificial antigens for sulfaguanidine and sulfathiazole[J].Journal of Hygiene Research,2010,39(4). |
| |
| Authors: | CUI Yanhua ZHANG Hailing MA Ying DAI Cuihong CUI Jie |
| |
| Abstract: | Objective To prepare artificial antigens of sulfaguanidine and sulfathiazole for detecting sulfaguanidine and sulfathiazole in foods of animal origin.Methods The artificial antigens were synthesized by the diazotization methods of coupling sulfaguanidine and sulfathiazole with bovine serum albumin ( BSA),and the coupling ratio was 10 ∶ 1 and 9 ∶ 1 respectively.The anti-serum of Balb /c mice was obtained after 5 injections of artificial antigens and the antibody titles were detected by iELISA method.Results The artificial antigens were identified by infrared spectra,ultraviolet spectra and immunized experimental animals.Conclusion The antigens for sulfaguanidine and sulfathiazole were acquired successfully.The results have laid basis for the specific immunoassay of sulfaguanidine and sulfathiazole. |
| |
| Keywords: | sulfaguanidine sulfathiazole artificial antigen |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
